David Klebl

Postdoc @BriggsGroup @MPI_Biochem | PhD at @AstburyCentre @UniversityLeeds | Cryo-EM and cryo-ET

[Charlie Scarff @DrScarff]

These ‘movies’ of proteins in action are revealing the hidden biology of cells https://t.co/g7Fap3pUXd

[Sven Klumpe @svenklumpe]

Over 4 years in the making, I am excited to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells. A 🧵

[David Klebl @DavidKlebl]

@BolandLab_GE @stemuench @DrScarff Thanks Andi!

[David Klebl @DavidKlebl]

Really happy to finally share this work! Started some time ago by Howard White, I worked on this during my PhD with @stemuench and now completed by @DrScarff's group 🙂
https://t.co/8XCkYsbCLm

[Charlie Scarff @DrScarff]

Interested in how myosin generates movement? and how actin activates it? Check out our preprint: https://t.co/fBQfBwuik9 @UoL_LICAMM @AstburyCentre
Beautiful collaborative effort!!

[Sven Klumpe @svenklumpe]

The paper is now online in its revised form @naturemethods: https://t.co/cD8UHb8u7J . Many thanks to the anonymous reviewers and @rita_strack for the nice review experience. And shoutout to twitterless Oda, JP, and @CJOKaiser! It has been a fun journey. 🥳🥳🥳🥳🎉

[David Klebl @DavidKlebl]

Really enjoyed visiting Leeds for the #AstburyRetreat, and it was so nice to see everyone again @AstburyCentre!

[Paul Taylor @PaulfrYorkshire]

@DavidKlebl on fast time-resolved Cryo EM a fantastic talk including great science, falling cats 🐈‍⬛ 😆 and photos from Iceland @AstburyCentre #AstburyRetreat

[David Klebl @DavidKlebl]

@BolandLab_GE Thanks @BolandLab_GE! Hopefully we can share some more details on the methods soon

[Mass Spec Leeds @MassSpecLeeds]

We're hiring! This new role will be involved in all aspects of facility operation with a focus on working with users to advance structural MS and proteomics projects on our new Orbitrap Eclipse and established UHMR platforms. Find out more and apply at: https://t.co/e3QorLVOAH

[David Klebl @DavidKlebl]

@stemuench And it works! Even in 50% EtOH 🙂

[David Klebl @DavidKlebl]

I'm glad we could make a small contribution to this work: https://t.co/p4j6D0fNVK Very different from our usual samples... ⏱️-❄️🔬 of 💎 @stemuench

[Anna Higgins @AJHiggins12]

Check out our recent paper on direct extraction from the membrane with novel amphipols! With pandemic interruptions this has felt a long time coming, so happy to see it out 😁

[David Klebl @DavidKlebl]

@carlospmata46 @stemuench @FrankSobott Thanks Carlos!

[David Klebl @DavidKlebl]

Our new paper is online 🥳 We show how to prepare EM grids for a broad range of timepoints, to achieve time-resolved cryo-EM. @stemuench @FrankSobott

[Structural Biology @ActaCrystD]

D​avid P. Klebl et al.: On-grid and in-flow mixing for time-resolved cryo-EM #ElectronMicroscopy #TimeResolved #Dynamics @universityleeds @universityleeds ... #IUCr https://t.co/ZK9grgHRxl

[SPC Facility & Garcia-Alai Lab @EMBL Hamburg @EMBL @SPC_EMBL_HH]

How do middle coat adaptor proteins assemble and contribute to remodel the plasma membrane? Take a look at our manuscript in Nature Comms.
➡️ https://t.co/CoRboxrOhy
#endocytosis
@EMBLHamburg @FrankSobott @svergungroup @DavidKlebl @stemuench @SkruznyLab @CssbHamburg

[Rene Henderikx @HenderikxRene]

Our publication on the invisible hands of sample prep is now visible to everyone! Please check it out to demystify some of your challenges on:
https://t.co/YGhO44rjg4

[David Klebl @DavidKlebl]

@RSBon_Lab @FrankSobott @stemuench @MatthewFeasey @HeskethEmma @naranson That's right. This took a lot longer than we originally thought.. But I'm very happy with the result!

[Stephen Muench @stemuench]

Took a while to get around the preferred orientation of HSPD1 but good to see the hard work of @DavidKlebl and the team @MatthewFeasey @HeskethEmma @RSBon_Lab @naranson @FrankSobott finally out. https://t.co/rHq4O7Lh45

[David Klebl @DavidKlebl]

and here's a link to the final version, published a while ago @Structure_CP: https://t.co/hDxT8dRlJ7

[David Klebl @DavidKlebl]

Excited to see our paper on proteins at the air-water interface (almost) published! https://t.co/q3PEuAEAiZ

[David Klebl @DavidKlebl]

#WeNeedChimeraX

[RadfordLab @RadfordLab]

Excited about our review on flanking regions in amyloids! Published today in Frontiers in Neuroscience:
https://t.co/9A8lMGb2tZ

[David Klebl @DavidKlebl]

@soph_hesk @biorxivpreprint @Javi_Lizaendo @embl @SkruznyLab @svergungroup @stemuench @FrankSobott Thanks Sophie!

[David Klebl @DavidKlebl]

Our work on endocytosis adaptor proteins is now on @biorxivpreprint 🥳 Great collaboration with @Javi_Lizaendo, @embl, @SkruznyLab and @svergungroup. And with @stemuench and @FrankSobott, of course!
https://t.co/33ZuGjoPTh