Jim Horne

Postdoc in the Luisi group at Cambridge Biochem working on the structural biology and biochemistry of bacterial efflux pumps.

[Jim Horne @_JimHorne]

@AdrianoAguzzi Why do you think higher glycerol is worse? My hypothesis would of been it is a better cryoprotectant

[Jim Horne @_JimHorne]

@SujeetKumar_LSU Cool results, I was asking @Figlegend about this very question last week!

[Jim Horne @_JimHorne]

New paper from @SujeetKumar_LSU and co, finally finding the OM clamp for one of the non-TamB AsmA-like proteins! If we could just do all vs. all cell envelope proteins in AF2 I wonder what else we would discover

https://t.co/QfYPlXxrhR

[Jim Horne @_JimHorne]

@maru_to_y 私も!

[Jim Horne @_JimHorne]

This is insanely cool. Rendering protein/cell scenes and tomograms using Unreal Engine 5 so you can display more with less GPU overheads and control a character to fly through them!
https://t.co/sKN5lGiKMJ

[Arne Janssens @ArneJanssens95]

More than proud to see our work on SlyB out in press
@Nature!

Who would have thought a small membrane protein could form protective nanodiscs. A big thank you to all collaborators and reviewers.

Find out more: https://t.co/iCXkf7G8r0

[Jim Horne @_JimHorne]

@4rtsy2wo @martinmbauer *postdocs are sub 4-years

[Jim Horne @_JimHorne]

@4rtsy2wo @martinmbauer Most postdocs across the UK including Oxbridge (except London) won't pass the threshold until at least postdoc year 3-4. I would suspect that >50% of all postdocs have are sub 4-years. So it would cover the vast majority of them

[Jim Horne @_JimHorne]

@KyrieGrasekamp @NatureComms @ChrisBel70 @SGribaldo @BasileBeaud Very cool stuff

[Jim Horne @_JimHorne]

Glad to see this paper from Sam finally out with tons of very cool in vivo fluorescent labelling tricks!

[Jim Horne @_JimHorne]

@ATinyGreenCell That was fun! Thanks for sharing

[Jim Horne @_JimHorne]

@sokrypton @g_pavlopoulos @BSRC_Fleming @kyrpides @jgi @SiruiLiu_ @UWproteindesign Very cool! I wonder if we can now visualise effectively 100% coverage for natural fold space or whether there are more hidden (to science) clades with many totally novel folds.

[Jim Horne @_JimHorne]

@xlinkx_pd @Chimera I've used this and it was very helpful! Was anything new added or just fixes?

[Stephen Fried JHU @fried_lab]

We're excited to share new tools, using photo-crosslinking mass spec 💡⚔️⚖️ to study protein structures and complexes in situ with high spatial and temporal resolution. Out now in @AmerChemSociety @an_chem. Short 🧵 1/8
https://t.co/Fl5NYw04A2

[Jim Horne @_JimHorne]

@bradyajohnston When are you talking in Cambridge?

[Jim Horne @_JimHorne]

@ATinyGreenCell Depending on how long you ran it for, I wonder if you would eventually select for a reduced lag phase?

[Shio Lab@Univ. of Miyazaki @shio_lab_UoM]

Our new paper published in
@jbiolchem.
Categorization of Escherichia coli Outer Membrane Proteins by Dependence on Accessory Proteins of the β-barrel Assembly Machinery Complex https://t.co/aCKJpKjmPY

[Pliotas Group @PliotasGroup]

In our just accepted paper in @angew_chem with @RadfordLab & @naranson in @AstburyCentre we used In-Cell PELDOR/DEER complemented by CryoEM to show the BAM complex adopts a lateral closed conformation in E coli cells in presence of antibiotic darobactin-B: https://t.co/XAWdh6ORXK

[Jim Horne @_JimHorne]

@michael_nielsen I don't if you have to completely retrain from scratch or if you can just update it by learning from mistakes. If it's a case of retraining it will be interesting to see what companies deem too expensive for a set return (is $1m for 5% better worth it?).

[Jim Horne @_JimHorne]

@michael_nielsen As you point out though, AF2 can give you local confidence measures which is in some ways even better than experimental work. We often fool ourselves that proteins are fixed constructs (XRC and EM just give a snapshot). In reality they can be dynamic and part of an ensemble. 3/3

[Jim Horne @_JimHorne]

@michael_nielsen I think you've got the answer in your thread pretty well. This is a predictive algorithm. Something of this accuracy has until recently been very rare for biology but physics has tons of equations that allow you to predict the outcome before doing the experiment. 1/n