Broggi's Lab 🧑‍🔬

Offficial Twitter account of the team "Innate Immunity at mucosal sites" at the Centre d'Immunologie Marseille Luminy (CIML)

[Broggi's Lab 🧑‍🔬 @AB_Lab_CIML]

and all the amazing people at @BostonChildrens @harvardmed

[Broggi's Lab 🧑‍🔬 @AB_Lab_CIML]

Last but not least thanks to everyone involved, starting from the @Lo_Zanzi, amazing mentor, the once again amazing @BennieSposito, the first postdoc of the @AB_Lab_CIML Julien Mambu, Philippe Naquet and Franck Galland @CIML_Immunology, @scott_snapper, Jay Thiagarajah

[Broggi's Lab 🧑‍🔬 @AB_Lab_CIML]

Using ZBP1 KO organoids and shRNA KD of GSDMC we could define that both are required fo IFN-λ induced lethality. Amazingly both GSDMC, ZBP1 and CASP-8 as well as Interferon responsive genes are upregulated in #IBD patients, and GSDMC levels correlate with #IFN responses!

[Broggi's Lab 🧑‍🔬 @AB_Lab_CIML]

Here's the first pre-print of the @AB_Lab_CIML, joint with @Lo_Zanzi lab. #IFNs are awesome but they can have a dark side. We show tath IFN-λ can delay tissue restitution in the gut by favoring epithelial cell death by #pyroptosis. @CIML_Immunology #IBD https://t.co/WwVlBK7rnw

[Broggi's Lab 🧑‍🔬 @AB_Lab_CIML]

Mechanistic studies in intestinal organoids recapitulated in vivo observations on gut epithelial cells showing that IFN-λ induces the upregulation of ZBP1, the activation of CASP-8 and the cleavage of the pore forming #gasdermin GDMC culminating in pyroptotic cell death.

[Broggi's Lab 🧑‍🔬 @AB_Lab_CIML]

We show that IFN-λ administration to mice recovering from colitis or radiation damage severely delays tissue restitution. Blocking endogenous IFN-λ on the other hand favors tissue restitution. IFN-λ in mice diminishes the number of proliferating intestinal stem cells.

[Achille Broggi @achillebro]

Happy to share our paper analyzing the landscape of #IFNs and #IFN responses in the respiratory tract of #COVID19 patients. IFNs in the upper airways drive mild disease while high IFNs in the lower airways correlate with severe symptoms. https://t.co/fMd3uU8Hkv