Gyanu Lamichhane

Interests: Bacteriology and antibiotic resistance. Apolitical. I see glass quarter full.

[Gyanu Lamichhane @BacteriaLab]

It is us (humanity) vs. the bad bugs that harm us. Let's think creatively and support each other. When necessary let's collaborate to attain synergy in our efforts.

[Gyanu Lamichhane @BacteriaLab]

Drs. Story-Roller and Pankaj Kumar from our group have led multiple studies providing both molecular mechanistic basis and evidence for efficacy of dual b-lactams vs Mycobacterium abscessus. Here are our publications: https://t.co/Srxxo9zHfm

[Natasha Spottiswoode @NSpottiswoodeMD]

Reading up on dual beta lactam therapy for Mycobacterium abscessus and found this paper and this gem: "Treatment of M abscessus infection presents significant challenges and is considered a Sisyphean task for many infectious disease clinicians."
https://t.co/NlmVjXSqUE

[Erik Solheim @ErikSolheim]

Heaven is a myth, Nepal is real!

Sunset on a warm day at the lake Phewa 🇳🇵❤️💙💚🧡

[Gyanu Lamichhane @BacteriaLab]

Shout-out to @NTMinfo for representing NTM patients and bringing their stories to those of us on the bench and at the bedside.

[Gyanu Lamichhane @BacteriaLab]

The Colorado Mycobacteria Conference 2022: Good to see an increasing number of talented investigators devoted to learning about mycobacteria and defeating the diseases they cause. Thx #NTM_CSU for hosting us.

[Gyanu Lamichhane @BacteriaLab]

Best of luck to new trainees who have joined this effort. I am sure all of us are united to help you succeed.

[Gyanu Lamichhane @BacteriaLab]

A good example of a new frontier. Exciting.

[Kremer Laurent @KremerLaurent1]

Successful phage therapy against drug resistant M. abscessus in cftr zebrafish. Mastered by
@mattdjohansen. Fantastic collaboration with @GHatfull and @DedrickRebekah. Thanks to @vaincrelamuco for funding.
https://t.co/dyIcTnTrCS

[Gyanu Lamichhane @BacteriaLab]

Very exciting concepts at the interface of chemistry and infection biology that focus on unexplored yet fundamental aspects of bacterial cell wall.

[Jorik van Rijn @JorikvRijn]

Below a cool teaser of what the microscopy setup from our latest preprint can do. Here, a Salmonella bacterium (arrow) divides on the surface of human intestinal cells. It then invades, and the epithelial cell reacts by instantly killing itself.

[Gyanu Lamichhane @BacteriaLab]

@Sandeepdhungana Good thinking. I am not sure if there is negative pressure in the tube that is anchoring the neutrophil.

[Gyanu Lamichhane @BacteriaLab]

Thank you for sharing this. A+ grade evidence of sensing and phagocytosis by a neutrophil. And also of cell polarity and kinetics of cytoskeletal rearrangement to achieve motility. Anyways, how is the neutrophil anchored to the glass tube?

[Yoni - יוני @Primary_Immune]

📽️🔬
An immune system "neutrophil"
senses its prey, then engulfs and kills it

credit: Heinrich Lab; Journal of Cell Science

[Gyanu Lamichhane @BacteriaLab]

Indeed please join the effort. Antibiotic development/discovery is one of the main challenges of our times as several bugs have become resistant to many Abx we have. A good blog to read more about the need for new Abx is https://t.co/OVz5JVFF2N.

[Freel Meyers Lab @FreelMeyersLab]

Come work on antibiotic discovery in the @FreelMeyersLab lab! Postdoctoral position available, please send your CV to Dr. Caren Freel Meyers at [email protected].
@Chemjobber

[Gyanu Lamichhane @BacteriaLab]

This series has been a go-to reference for many protocols we use. Thank you for putting these together.

[Prof Tanya Parish @ProfTanya13]

The fourth edition of Mycobacteria Protocols is now out. We thank all the authors and hope it benefits the research community as much as the previous editions. https://t.co/NfVG3ZUGHE

[Gyanu Lamichhane @BacteriaLab]

Indeed an interesting microbe. Amazing heterogeneity in geno- and phenotypes. A bit more finicky compared to other mycos. Learned much and continuing to learn from this myco..

[Kremer Laurent @KremerLaurent1]

Mycobacterium abscessus, the Microbe of the Month in Trends Microbiol.
https://t.co/m4pVLi6ic1

[Gyanu Lamichhane @BacteriaLab]

@pmoyniha @GlcNAcMurNAc I agree with you. We have discussed potential reasons for differences in our observations in the discussion section. Since Tn mutant pool is an outgrowth pool, it is inherently difficult to detect growth defective mutants such as this. But Tn mutants are powerful first screen.

[Gyanu Lamichhane @BacteriaLab]

An interesting protein with domains reminiscent of a classical PBP, but it also exhibits a strong b-lactamase activity. M. abscessus mutant lacking this protein grows as a single clump and is wiped out from the lungs of mice...Thx @GlcNAcMurNAc for sharing this.

[PeptidoglycanPapers @GlcNAcMurNAc]

Glby, is a PBP with β-lactamase activity and is required for in vivo viability of M. abscessus https://t.co/E6g07yFeOc

[Gyanu Lamichhane @BacteriaLab]

@FreundlichGroup Thx Joel. A particular finding reported in our 2017 NCB paper, that penems react more strongly with LDTs catalyzed this project. I collaborated with you for several studies reported in that paper. That was also an excellent team effort.

[Gyanu Lamichhane @BacteriaLab]

Our team has taken one step towards addressing the challenge of developing new #antibiotics. Early results show promise but much work lies ahead to develop one that is useful bedside. We're determined to fight against #AMR, #TB and #mycobacteria. Our paper https://t.co/CYZcOoUYon

[Gyanu Lamichhane @BacteriaLab]

@wfwrighID Dr. Wright, Thank you for your service, everything you have done to help others and continue to do. Immense respect to you.

[Gyanu Lamichhane @BacteriaLab]

Dear graduate students, let this sink in. If you want to read more about what Albert did, I recommend the novel 'the greatest story never told' by Frank Ryan. Just like Albert's, your contributions are much needed and will be valuable to humanity.

[Microbiology Society @MicrobioSoc]

#OnThisDay in 1943, graduate student Albert Schatz isolated streptomycin, the first antibiotic found to treat #TB. Find out more about #tuberculosis in our blog post: https://t.co/UMZd1iiQ9a

[Gyanu Lamichhane @BacteriaLab]

@ProfTanya13 @GTsodikova @AcademicChatter While I fully support those who can manage paperless journal and lab notes, I still keep handwritten notes and thus agree with @ProfTanya13 and @GTsodikova. Attached is a picture of lab notes of a little assay I did today.