Our new preprint optimising search parameters in DIA is out. We show DIA suffers effects similar to match-between runs in DDA, with false positives detected across runs.
But we offer simple solution that dramatically reduces them while maintaining proteome depth. #proteomics
Protocol Alert!
Want to generate deep immune cell proteomes? Together with @AJMHowden @AJ_Brenes @metab_innate @SimonAr09230172 and the team, we’ve developed a step-by-step protocol for macrophage proteomes-applicable to a range of cells.👉https://t.co/RQ6p8BWPcA @STARProtocols
Included in the protocol: we compare DIA and TMT proteome coverage from https://t.co/XTGTu2bMh8 @metab_innate and discuss the depth our DIA method achieves.
How thrilling to realize the mycology community is so supportive and genuine. Thank you #HFP2022 for the opportunity to present my research, thanks to the community for the feedback—can’t wait to try these suggestions!