Quick! Abstracts closing for ASDS in August - deadline 23:59 AEST 14 April 2024
Theme e.g.
-Breeding, genetics & resistance
-Diagnostic developments
-People & soil: social, cultural, educational dimensions
-Soil health
-Disease management
https://t.co/Nu0dArhrWZ
@LPerepolkin It's always good to make sure, so that you are certain that when you send of the amplicon for sequencing you'll be getting back nematode DNA. Nothing worse than paying for barcode sequencing and getting fungal sequence data back!! Good luck, keen to see what you find! 2/2
@LPerepolkin Nicely done Lawrence! May I suggest including a negative control in your PCRs? This will confirm that none of your reagents are contaminated. Depending on what gene region you are targeting for amplification, the primers you use can sometimes amplify non-target DNA 1/2
Had a great time presenting my work on root-knot nematode diagnostics at the #2023APPSAdelaide conference. A lot of really thought provoking presentations and posters at the conference.
@LPerepolkin Are you finding you can discern more features when they're stained? I used to stain trematodes for morphological characterisation, but thus far have found nematodes to be easy enough to study without stain