Have anyone seen this peak in PRM mode? First figure is skyline file Light/heavy. Second is from Xcalibur TIC/Light/Heavy. where should I change MS setting to make it like normal profile peak?
#Skyline#PRM#targetedproteomics
I ran 27 tips with spike-in heavy peptides by 88mins. I put 27 tips on the #Evosep at once.
After 12 hours, the intensity of more than half heavy peptides were drop > 70%. First figure is the not decreased heavy and the 2nd figure is the decreased.
Any suggestion?
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