Here's a new and exciting @nanopore paper we have helped with led by @ferencmueller and his group in @CellCellPress. In this, we examined the formation of the transcription body in Zebrafish, specifically the mir-430 region:
https://t.co/i31MEiBSiC
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So @nanopore to the rescue, with a ultra HMW prep on our P24, using R10.1 chemistry ably performed by @GenomicsB and then a denovo assembly using Canu on @uob_bear HPC (a 2TB node!) with an N50 of 110kb and 44x coverage.
Another toy (serious science equipment) for @GenomicsB this time courtesy of @MissionBio via our BBSRC Grant - if you want to do single cell DNAseq get in touch!