Guido van Mierlo

Assistant Professor @ the department Medical BioSciences in Radboudumc. Interested in quantitative (prote)omics, tumor immunology and interindividual variation.

[Molecular Cell @MolecularCell]

Not all CBXs are created equal: A role for the disordered central region of CBX4 in pediatric glioma https://t.co/t4SUBQY72P

[Nature Biotechnology @NatureBiotech]

Induced proximity at the cell surface - @CarolynBertozzi https://t.co/tgtdWuTzqp

[Nature Biotechnology @NatureBiotech]

Toward single-molecule protein sequencing using nanopores https://t.co/kgokDJlynO

[Cell Reports @CellReports]

PRC1 and PRC2 proximal interactome in mouse embryonic stem cells https://t.co/veDmRHMqBs

[Guido van Mierlo @GuidoMierlo]

Excited to announce that as of this week, the van Mierlo lab is officially open in the dpt. of Medical BioSciences, @RIMLSInstitute , @radboudumc Nijmegen. Quantitative (prote)omics, tumor immunology, and a bit of epigenetics. Exciting times ahead #newPI .

[Guido van Mierlo @GuidoMierlo]

Our preprint is finally there! We benchmark and showcase approaches for inferring cooperation between cis-regulatory elements using epigenome data and explore the impact of non-coding variants. Great work with @OlgaPushkarev @BartDeplancke and team.
https://t.co/xNSZmTzeZ2

[Guido van Mierlo @GuidoMierlo]

Happy to share that our detailed protocol with @LabVermeulen for off the shelf proximity labeling without transfection or transduction using ProtA-TurboID is now online @NatureProtocols . As a bonus, we profiled the proteome of the centromere.
https://t.co/HdzyqilrFt

[Guido van Mierlo @GuidoMierlo]

Very cool example of how a non-coding variant can induce the activity of multiple regulatory elements in a locus through creating an ectopic TF binding site, finally leading to expression of the embedded Axin2 gene

[Nature Methods @naturemethods]

The @BartDeplancke lab and colleagues describe DisCo, a single cell analysis platform designed for low cell-input samples, which allows them to profile individual intestinal organoids and in vivo-derived small tissues.
https://t.co/GpficQA16H

[Guido van Mierlo @GuidoMierlo]

@macro_momo Not sure. But you could maybe look at the work from the Krijgsveld lab on protease-resistant strep (rafiee, molsysbio, 2020). in any case, for us non-mag beads were always cleanest in MS applications using on bead digest, Both AP-MS and BioID based

[Guido van Mierlo @GuidoMierlo]

@macro_momo On bead digestion always worked well for us, but make sure to use streptavidin sepharose / agarose based beads and not the magnetic ones, those give a lot of contaminants

[Guido van Mierlo @GuidoMierlo]

Arrived in Santander for the #EMBOenhanceropathies workshop. Great location (view from the venue here) with an exciting programme ahead!

[Guido van Mierlo @GuidoMierlo]

Want to perform proximity labeling without having to transfect or genetically manipulate cultured or primary cells? We provide a flexible off-the-shelf enzyme that can be used in combination with a specific antibody of choice. Plasmids/constructs available to those interested!

[MVermeulenLab @LabVermeulen]

Check out our latest paper in which we developed a new 'off the shelf' proximity biotinylation enzyme, called ProtA-Turbo, that facilitates proximity biotinylation experiments without the need for genetic manipulation of target cells.

https://t.co/mgeiZWShO2

[Guido van Mierlo @GuidoMierlo]

@da40cambs I always did 1 hour RT, 30 minutes if really in a hurry

[Guido van Mierlo @GuidoMierlo]

Ex utero grown mouse embryo's up to E11, awesome work from the @jacob_hanna lab in @nature. Get those crispr and compound screens going! https://t.co/lcvsdSgw1L

[Guido van Mierlo @GuidoMierlo]

Interested in proteins forming condensates? Together with @LabVermeulen and @svheeringen we provide a machine learning classifier that provides a phase separation likelihood for every protein in a given proteome. Happy to see this out @CellReports
https://t.co/IXZbFUKgPq

[Guido van Mierlo @GuidoMierlo]

If you are doing immunology-related research in Switzerland, this could be a very interesting new initiative to check out! @syis_ecr @y_efis

[Jorieke Weiden @joriekeweiden]

Are you a young immunologist with a link to Switzerland? Connect with the SYIS to bring together #ECR! @syis_ecr @y_efis #network #immunologists

[Guido van Mierlo @GuidoMierlo]

@MarvinTanenbaum Maybe you could attach biotin molecules to PIC hydrogels? Haven't used these, but they display temperature-dependent gelation

[Gert Jan C. Veenstra @GJCVeenstra]

Two exciting faculty positions in our institute @Radboud_Uni in

Computational Biology
https://t.co/1YaZ9wMjFl

Cell Biology
https://t.co/aMDPyKhorm

The job adverts are targeted to junior faculty, but more senior candidates are encouraged to respond

RT=nice

[Rob Klose @RobKlose]

⁦Latest story from lab led by @EcoRInya⁩ ⁦with ⁦@ahturberfield⁩ @blackledge_neil⁩ ⁦@PaulaDobrinic⁩ ⁦⁦@mileshuseyin⁩ ⁦⁦@AnnaLastuvkova⁩. New insight into pervasive H2A ubiquitylation and transcription. Well done team! https://t.co/E0ET315BVm