Sean Halper

SynBio PhD, Salis Lab Alumni

[Ayaan Hossain @bioalgorithmist]

Very happy to announce the publication of Synthesis Success Calculator! Hearty congratulations to the fearless and amazing first author @halper_1! Paper: https://t.co/XxB56WRfQ8

[Howard Salis @hsalis]

Super Hat Tip to @bioalgorithmist who worked tirelessly on this massive design problem w/o much sleep. As always, thanks to @alexcampreis for help with conception, protocol dev, and schematics and to @SarthokR for cross-strain validation.

[Howard Salis @hsalis]

FOR IMMEDIATE RELEASE & USAGE: "A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples" Protocol at https://t.co/x2c2v8uvw3. Primers & Spike-in Controls at https://t.co/9cWzQrfz5P

[EBRC @EngBioRC]

Interested in designing your own Extra Long sgRNA arrays? Check out their ELSA calculator here: https://t.co/lnlXjSGc07 @hsalis Lab and @DeNovoDNA

Have a great #ResearchThuRsday and please reach out if you'd like to get featured yourself!

[Howard Salis @hsalis]

Hat tip to @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist @phillip_clauer for their great work!

[Sean Halper @halper_1]

@alexcampreis 🍸

[Nature Biotechnology @NatureBiotech]

@PennStateChE @PennStateBME @BioinformPSU @hsalis @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist Science behind the scenes: Multiplexed CRISPR and sgRNA arrays with the @hsalis Lab #NBTintheNews via @NikoMcCarty @PLOSSynbio https://t.co/Pm2Zke0jPb

[Ayaan Hossain @bioalgorithmist]

@NikoMcCarty just published a nice behind the scenes interview of my colleagues @alexcampreis, @halper_1 and my boss @hsalis on our recent ELSA paper. https://t.co/D3r698AQKw

[Nature Biotechnology @NatureBiotech]

The November issue is live https://t.co/Q6CurQKIFk

[Nature Biotechnology @NatureBiotech]

@PennStateChE @PennStateBME @BioinformPSU @hsalis @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist Co-authors @alexcampreis, @halper_1 & @hsalis go #behindthepaper to show how adding many-site targeting to CRISPR will have profound biotech implications https://t.co/bCOJHqISUl

[Sean Halper @halper_1]

Open access link to the ❄️ELSA❄️ paper for anyone stuck behind the paywall: https://t.co/66ZrgfBMV2

[Howard Salis @hsalis]

Our work on Extra Long sgRNA Arrays (ELSAs) began during a free-wheeling graduate course. After designing several CRISPR-based circuits, we found many repetitive DNA sequences (a big #SynBio challenge). Learn more in this #NatureBiotech #BehindThePaper: https://t.co/Wmjmx5A7OJ

[Sean Halper @halper_1]

@alexcampreis double feature with the JLo CRISPR show(when that comes out)?

[Amy Maxmen, PhD @amymaxmen]

New Netflix series on #Crispr runs with the idea that anyone w/a pipette can make super humans in their garage 🙄. I've only seen the trailer but cringing #scicommgonewrong https://t.co/G7OmfgmY30

[Jake Wintermute 🧬/acc @SynBio1]

My new startup leverages CRISPR-Cas gene editing technology and proprietary machine learning algorithms to develop super long pumpkins (spookier).

[Setoh🍥 @yxsetoh]

This is a CRISPR-cas9 mediated deletion (IFNAR1). Done by my undergraduate student 🙌, in < 4 months 🙌, setting up everything from scratch 🙌, using resources from @zhangf @Addgene. Amazing sharing economy in research. Sorry, had to share this super proud supervisor moment.

[Be the change you want to be @AgBioWorld]

At a synthetic biology conference, a food truck handed out GMO papaya and yogurt samples to hundreds of attendees. “A lot of folks in our world aren’t using the term ‘GMO,’ and instead will say it’s something like ‘gene modification’ or ‘CRISPR’”

https://t.co/evJDuTz97Y

[Sean Halper @halper_1]

👀 https://t.co/qg05aXOalT

[Jose Utrilla @joseutrilla]

Super useful for proteome and host engineering, its amazing how fast CRISPRi is moving #synbio

[Howard Salis @hsalis]

In a nutshell: Pick your targeted genes (up to 20). A designed ELSA regulates them (repression shown). Fully non-repetitive sequences ==> easy to synthesize & assemble. Genetically stable & evolutionary robust, great for creating selective (growth-inhibiting) phenotypes.

[Tony Nolan @TonahN]

@s_verkuijl I read this. I think one of the most interesting things is that they show is that Cas9 quickly can become limiting in the formation of active RNPs when there are competing guides around. Something we have suspected but never proved.