Top Tweets for #askZebrafish
π€ #AskZebrafish: Does anyone have experience extracting (tissue-specific) cell monolayers for culture from ZF embryos? Looking for a contact to pick their brain.
π€ #AskZebrafish: "Do any of you have a protocol or a kit recommendation to do subcellular fractionation of zebrafish embryos. Ideally I'd like to separate the mitochondria and the nucleus (and as a bonus the rest of the cell parts)"
Time for an #AskZebrafish for the community that everyone can help answer!
What's one thing you wished someone told you about working with #zebrafish (or your favorite fish model)?
We'll go first: You will get wet ππ―
π€ #AskZebrafish: βWondering if anyone has recommendations on a relatively inexpensive device to measure water flow rates in a swim tunnel?β
π€ #AskZebrafish: Looking for a zebrafish version of the Mito-RiboTag mouse line. Has anyone made one or might have a vector they could share? Any leads would be thoroughly appreciated!
π€ #AskZebrafish
Does anyone in Europe have Tg(UAS:iGluSnFR) and Tg(UAS:GCaMP) zebrafish lines available? Looking to share or obtain embryos if possible. Thanks in advance! ππ¬
@ZebrafishRock
#Zebrafish Classifieds π Looking for someone(s) with a shared core facility that could share any information about different price models that are out there (e.g. costs/day/tank; services such as breeding, raising fry, transgenesis, etc.). DM with contact email π #AskZebrafish
π Query from Slack: Looking for one of the following immune reporter π lines in πΊπΈ (Bonus points for being close to Boston):
irg1:EGFP
tnfa:EGFP
arg2:EGFP
#AskZebrafish
This query continues to come up π§ Looking for a reliable stock of microinjection needle loading tips? Users have found issues regarding ones from Calibre Scientific (significant backlog) and Bio-Rad (discontinued?). #AskZebrafish
π€ #AskZebrafish
Neuro tweeps: are there mRNA expressed in neuronal axons (not cell bodies) that are markers of axonal degeneration/regeneration? Extra bonus if it's in zebrafish. Please RT and gracias!
Well... it's getting harder and harder to stay on this platform. For this weekend's #PollForTheShoal, where do we go from here? #AskZebrafish #RockTheVote
Query from Slack: Looking for someone experienced with pre-extraction of soluble/unbound proteins of #zebrafish embryos (<6hpf) for IF? Would love to hear tips about buffers and protocols that worked. #AskZebrafish
π€ #AskZebrafish
Twitterverse, help me out: I remember a paper reporting the use of a redox (H2O2) biosensor (possibly one of the Hypers) in zebrafish larvae, showing high activity at the posterior end and, I think, in the PSM & somites. But obviously can't find it anymore... @ZebrafishRock
Letβs try a beginning of the week #PollForTheShoal π€ We are asking #zebrafish peepsβ¦ In light of an opinion piece from @PNASNews, do you record or account for time-of-day in your fish experiments? #AskZebrafish #RockTheVote
Query from Slack: Does anyone have the olig1 or 2 promoter in p5E (Gateway system) and would be willing to share it? Any help and/or leads would be appreciated! π #AskZebrafish
Another query from Slack: Looking for an oxytocin antibody that works for IHC in zebrafish? The one used by @carolineleiwee & @douglasslab has been discontinued by Biorad π #AskZebrafish
After a couple months of poor engagement, we could use some feedback π Do you click on the jobs and research links in the #DanioDigest? #RockTheVote #AskZebrafish
Query from Slack: Has anyone used the NANOLITER2020 INJECTOR from WPI for embryo injections? Does it work well? An absolute garbage? Works better or similar to the PV850? Prices? The person had previously used Nanoject 3 from Drummond but it is not sold in India. #AskZebrafish
Query from Slack π Looking for TgBAC(cd4-1:mCherry)^umc13 and Tg(lck:lck-EGFP)^cz2 swimming around in πΊπΈ #AskZebrafish
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