Bacillus megaterium phage G

about to get a big head from this (~ 180 nm in diameter at the 5-fold vertex and packaged genome length 626 kbp)

[Bacillus megaterium phage G @megaphageG]

Happy Monday!! Check out this #ussie of me and my phage g buddies, the true O(phage)G’s!
https://t.co/Avf9SgpIy0

[Bacillus megaterium phage G @megaphageG]

González, B., Monroe, L., Li, K., Yan, R., Wright, E., Walter, T., Kihara, D., Weintraub, S. T., Thomas, J. A. (This 2020). Phage G Structure at 6.1 Å Resolution, Condensed DNA, and Host Identity Revision to a Lysinibacillus. Journal of Molecular Biology, 432(14), 4139–4153.
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[Bacillus megaterium phage G @megaphageG]

This image is mad colorful! But what it really is a Phage G major capsid protein hexamer and decoration protein trimer arrangement. This image shows the structure gp27 hexamers and gp26 trimers positioned at the 3-fold axes around the hexamers. Feeling #twsited !

[Bacillus megaterium phage G @megaphageG]

You couldn’t find me at the bottom of the barrel, I’m a top of the barrel kinda bacteria. I’m aerobic! 🌬️

[Bacillus megaterium phage G @megaphageG]

Cooney, P. H., Jacob, R. J., & Slepecky, R. A. (1975). Characteristics of a Bacillus megaterium Bacteriophage. Journal of General Virology, 26(1), 131–134. https://t.co/glSKllCaEk
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[Bacillus megaterium phage G @megaphageG]

What do trees and I have in common? We both came from soil! 🌴

Bacteriophages were isolated from the soil using Bacillus megaterium atcc 19213 as host for isolation, propagation, and assay. By growing Nutrient Broth (Difco) cultures on B. megaterium with 2 to 6 p.f.u./bacterium

[Bacillus megaterium phage G @megaphageG]

https://t.co/2o2032eAhZ

[Bacillus megaterium phage G @megaphageG]

Ackermann, Hans-W. (2009). Create new species named Bacillus phage G to be unassigned in the family Myoviridae [Review of Create new species named Bacillus phage G to be unassigned in the family Myoviridae]. ICTV, 2009.010a,bB, 1–6.

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[Bacillus megaterium phage G @megaphageG]

#selfiemonday , selfie of me stretching out and showing off my length! Check out my tail, "Tails measure ~410 x 20 nm in the extended state, are surrounded over their complete length by a single spiral fiber, and carry a bundle of indistinct, curled terminal fibers". Source below

[Bacillus megaterium phage G @megaphageG]

Feeling #blessed for the ocean bc cobalt is my fave color! 💙 I require Co2+ for optimal propagation, so it just makes sense, okrrr!

Source:
https://t.co/RCzzm48Jpa

[Bacillus megaterium phage G @megaphageG]

they say there may be a map on the back of the Declaration of Independence, but what they don’t tell you is it’s a genome map ;) 626 kbp in length.

- Nicolas Phage

Source: https://t.co/b8Xzqq27Rn

[Bacillus megaterium phage G @megaphageG]

some of the other phages wanted to DNA wrestle but they can’t beat me, the molecular weight of my double-stranded DNA (dsDNA) genome is 4.9×108 daltons! Can’t beat that! 🧬💪🏽

Source:
https://t.co/zPUWgiAKMi

[Bacillus megaterium phage G @megaphageG]

Feeling : #found #seen #heard

TBT 1968 when Gianfranco Donelli reported the isolation and initial characterization of ME (Bacillus megaterium bacteriophage named phage G).

Thanks Don :)

[Bacillus megaterium phage G @megaphageG]

Some say I’m basic phage but I say I’m neutral. (Greatest stability between pH 7-8) 🧪⚛️

[Bacillus megaterium phage G @megaphageG]

If there was a competition of which phage is bigger I would def win : I’m reported to have the largest genome of all discovered Myoviridae with nearly 700 protein-coding genes. 🧬 💪🏽