@fungalhyphae@AndorTechnology@ImarisSoftware Mainly that the full resolution image of the file pyramid was missing/corrupted.
If you can still see r0 of the image in Fiji, I think you should be able to resave the image. Maybe as a tiff and then reconvert to ims.
Hard to say though without access to the data or imaris ๐คทโโ๏ธ
@fungalhyphae@AndorTechnology@ImarisSoftware Do the unstitched tiles look ok? Is this the same problem you were having before? I seem to remember I was able to partially recover the data by running it through the file converter but there was something inherently wrong with how fusion had written the ims file. 1/2
Iโll be representing Imaris on the Andor booth along with our product manager! Come say hello!
Have an Imaris question or problem you want help with? book some time with me :
https://t.co/sHnRwAF8eG
#elmi2024#rms#bioimaging@RoyalMicroSoc@CCI_liv
Visit Oxford Instruments Andor at elmi2024 in Liverpool!
Drop by Booth 22 and be the first to see our brand new Benchtop Microscopes in person, or check out our variety of workshops in Room 4B - https://t.co/QuEbAubGgO
See you there! #elmi2024
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Join us for our upcoming webinar โImaris 10.1 - Trainable AI Image Analysis for Everyone" on Thursday 7th December with Anna Paszulewicz!
Don't miss out and register your place today!โคต๏ธ
https://t.co/Lj2tMMYk6b
Are you interested in analyzing light sheet imaging data ?
Here's a memory from the Kusumbe lab
Junyu Chen, a postdoc at @KusumbeLab (now a group leader in China) training Lincoln Biswas in image analysis @Anjali_Kusumbe lab
@MaKaefer @preibischs @fungalhyphae@AndorTechnology@FijiSc@ImarisSoftware Obviously Iโd always recommend our stitcher for all your stitching needs ;) but if you want to open source stitch IMS files you can open them using bioformats in Fiji, then use one of the stitching tools. Big stitcher is a good tool in my experience for general mosaic stitching
I had great fun this week testing #AndorDragonfly@AndorTechnology with amazing specialistsย Ruediger Bader, Mark Browne and Andy Hubbard
And wow, doesn't this #gastruloid look like a spaceship ๐?
(gotta โค๏ธ the integrated 3D viewer power by #imaris as well!)
Anyone at @mmc_series in #Manchester this week? Come say Hi at the @OxInst booth! Iโll be talking about Machine learning in Imaris at the workshop sessions tomorrow if anyone is especially keen ๐
@fungalhyphae@FijiSc FYI you can reduce TS intensity changes in Imaris using โnormalise time pointsโ XT plug-in and it should work for BF.
Also using this tool, one of our supporters came up with a work around for flattening intensity differences between fields and image series for Batch analysis
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Credit: Rania Hijazeen
@KusumbeLab - @UniofOxford
Two days left to enter our image competition...
https://t.co/ruPJInGiZi
@MAG2ART Are these FUCCI markers, or a newer iteration? Such a useful tool for CC biology. I used these constructs a lot to define cell cycle timings for cell types. The aim was to correlate protein dynamics of inflammatory transcription factors to cell cycle phase. tl;dr We did.
@pablo_jack@DaniBeckman@zeiss_micro We do have a free viewer which you can do quite a lot of cool visualisations with if you know what youโre doing. Itโs in our website.