Andrew Klocko

Researcher working to understand fungal genome organization and function, including histone PTMs and gene expression. Tweets are my own.

[Neurospora crassa Community @NcrassaFungi]

Co-translational folding allows oxidative phosphorylation proteins to be inserted into the mitochondrial membrane. Now published in Mol Cell!
https://t.co/GGgAdExS8V

[Neurospora crassa Community @NcrassaFungi]

New work from the He, Liu, & Zhou labs details how oxi. phos. in Neurospora is controlled by mitochondrial translation. Here, the intrinsically disordered region of Mitochondrial rRNA Methyltransferase MRM1 binds ribosomes and mRNA to repress translation but allow co-tln folding

[Neurospora crassa Community @NcrassaFungi]

Find the manuscript here, newly published in the journal Molecular and Cellular Biology:
https://t.co/9QLUVLTtBz

[Neurospora crassa Community @NcrassaFungi]

Exciting work out of Eric Selker's lab shows that H3K27me3, catalyzed by the PRC2 complex, might be controlled by telomere repeats and the Telomere-repeat binding factor TRF-1. Ectopic telomere repeats allow TRF-1 binding, gain H3K27me3, and associate with endogenous telomeres.

[Neurospora crassa Community @NcrassaFungi]

Check this neat article here, in the Journal of Genetics: https://t.co/mA6NfifqO6 (https://t.co/1arRy72iBf)

[Neurospora crassa Community @NcrassaFungi]

Cool historical perspective from Durgadas Kasbekar about how in Neurospora Meiotic Silencing of Unpaired DNA (MSUD) was discovered. Here, a cross of a wildtype, euploid strain to a strain with a chromosome segment duplication gave mostly barren spores but a few spores survived.

[Neurospora crassa Community @NcrassaFungi]

Newly published in the journal Epigenomes: https://t.co/MPouFvIYLp

[Neurospora crassa Community @NcrassaFungi]

Nice new manuscript from the Shiu lab about how ARS2, a Cap Binding Complex member, is needed for Meiotic Silencing of Unpaired DNA, possibly by targeting Argonaute to mRNAs from unpaired genes. ARS2 interacts with the CBC and is needed for vegetative growth and sporulation. Cool

[Neurospora crassa Community @NcrassaFungi]

See the new work in the journal Epigenomes: https://t.co/4Lkw6x2QAn

[Neurospora crassa Community @NcrassaFungi]

New work from the Shiu lab shows the Neurospora Hsp70 chaperone acts in Meiotic Silencing of Unpaired DNA. In fruit flies, Hsp70 activates Argonaute to bind small interfering RNAs for silencing, and Sy et al. shows the Neurospora Hsp70 binds Argonaute and silences unpaired genes.

[Neurospora crassa Community @NcrassaFungi]

check out this work, which is a preprint on the BioRxiv: https://t.co/KiU2eUTo69

[Neurospora crassa Community @NcrassaFungi]

New work from the Kronholm lab examines epialleles in Neurospora. Here, a mutation accumulation experiment that bottlenecks strains tested if DNA or histone methylation (5mC or H3K9me3) epialleles occur. Here, centromeres rapidly gain 5mC epialleles but H3K9me3 is unchanged. Neat

[Neurospora crassa Community @NcrassaFungi]

Please see this new publication in FEBS Letters for more details: https://t.co/TAdQ1FEcTR

[Neurospora crassa Community @NcrassaFungi]

A new review from the Larrondo lab examines how the intrinsic disorder of circadian clocks enables temporal regulation. Using the Neurospora clock, they discuss how clock protein phosphorylation allows distinct binding partners across the day, despite poor sequence conservation.

[Neurospora crassa Community @NcrassaFungi]

Check out how this new work provides insight how developmentally identical cells coordinate their mutual attraction, now published in PLoS Genetics: https://t.co/PswNEwlodM

[Neurospora crassa Community @NcrassaFungi]

New work from the Fleißner lab explores hyphal fusion in Neurospora, where alternating recruitment of a MAP Kinase and the fungal protein SO mediate cell-cell communication. The protein EOP-1 enables this crosstalk and EOP-1 has oscillatory recruitment and acts upstream of MAPK-1

[Neurospora crassa Community @NcrassaFungi]

Check out this cool preprint!
https://t.co/rkXMe0I2k6

[Neurospora crassa Community @NcrassaFungi]

Another big week for the Dunlap/Loros labs: a BioRxiv paper suggests that nuclear import of FRQ by the Importin alpha protein is needed for proper circadian rhythms. Using FRAP, they show that FRQ import is slow (tens of minutes) as a rate limiting step for the circadian clock

[Neurospora crassa Community @NcrassaFungi]

Together, this work suggest that the Neurospora circadian clock, with both FRQ and WCC, is regulated by a "phoscillator" phosphorylation/dephosphorylation cycle!
Newly published in PNAS! See the paper here:
https://t.co/4vqqDVYvL4

[Neurospora crassa Community @NcrassaFungi]

New work from the Dunlap/Loros labs shows that the circadian transcription activator, White Collar Complex, is not only phosphorylated at dusk, but a small WCC pool is dephosphorylated in the AM, which initiates FRQ transcription so that WCC is repressed by FRQ phosphorylation.