WIBRFlow

Flow Cytometry Core Facility @ The Whitehead Institute for Biomedical Research

[WIBRFlow @WIBRFlow]

Did you miss the latest #OpenFlow session on DNA analysis by #flowcytometry? Check out the recorded version on our Youtube here: https://t.co/OvT36tjJhI

@CrickTraining @FlowMskcc

Endless thanks to Kuan-Chung Su from Iain Cheeseman's lab here @ WIBR for help with this class!

[flowMSKCC @FlowMskcc]

Happy #flowpostit Monday! Curious how to tackle #tandemdyes to obtain the most optimal conditions for your flow experiment? look no further 👇

@WIBRFlow #flowcytometry #flowedu #flowcyto

[WIBRFlow @WIBRFlow]

Still not too late to register! We'll be back at the instrument today (in 1 hour) to look at cell cycle by flow cytometry! Looking forward to seeing everyone.

[DerekDaviesCytometry @DDCytometry]

Todays the day! The first OpenFlow Cytometry webinar of 2022. At 18:00GMT join @FlowMskcc and @WIBRFlow and me as we look at DNA analysis by #flowcytometry - theory and practical - come armed with questions. Still not too late to register:
https://t.co/8GVscodSpX

[DerekDaviesCytometry @DDCytometry]

Just over a week till our latest #openflowcytometry webinar with @FlowMskcc and @WIBRFlow We will be discussing DNA analysis by #flowcytometry As usual it is free but requires registration:
https://t.co/8GVscodSpX

[WIBRFlow @WIBRFlow]

@CYTOMETRYWHIZ @FlowMskcc Happy New Year Rafael!! Hoping to see you in 2022!

[WIBRFlow @WIBRFlow]

The first #flowpostit of 2022 (in collaboration w/ @FlowMskcc) is here! Have you ever wondered about why laser delays are so important in your #flowcytometry experiments? Check out⬇️to get more information on what exactly these delays are and how they can impact your experiments!

[WIBRFlow @WIBRFlow]

Wishing all of our flow friends near and far a very happy holiday season!

[Whitehead Institute @WhiteheadInst]

Whitehead Institute's Whitehead Fellows Program gives early-career scientists the rare opportunity to set up their own research labs as an alternative to traditional postdoc positions. Applications are open now until February 1st — apply today: https://t.co/hWJcvgeOtC

[WIBRFlow @WIBRFlow]

Our next Flow Post-it in collaboration with MSKCC is out!

Have you ever seen spillover between fluorochromes you weren't expecting? This is a good resource to understand why it may be happening!

Happy Flowing!

[flowMSKCC @FlowMskcc]

#flowpostit Cross Laser Excitation - what can we do about it? 👇 - great collaboration w/ @WIBRFlow

more: https://t.co/6mDNUHgKjJ

[WIBRFlow @WIBRFlow]

@jessvazquez314 @GRFlowDoctor @cat_uoc (part 4) and here is a photo to show a similar circumstance and what the root cause was. Sorry for the long winded answers. Happy Flowing and good luck!!

[WIBRFlow @WIBRFlow]

@jessvazquez314 @GRFlowDoctor @cat_uoc (part 3) ..and look at an nxn plot of all fluors. See if there is a pattern of what is typically called "overcompensation" or "undercompensation" even though it's unmixing. You can then try and identify what the issue is through your controls as Laura outlines in her post.

[WIBRFlow @WIBRFlow]

@jessvazquez314 @GRFlowDoctor @cat_uoc (part 2) I suggest looking at your single color controls and doing an overlay to see if your controls are as bright or brighter than your experimental samples. If not, that's a problem! You can also look at this this data in a third party analysis software (or in SpectroFlo)...

[WIBRFlow @WIBRFlow]

@jessvazquez314 @GRFlowDoctor @cat_uoc (part 1) Chiming in here as well! @cat_uoc posted a great resource. Specifically I would be curious about (how Laura mentions) if your single color controls are as bright or brighter than your experimental sample. If not, it can lead to issues with unmixing.

[WIBRFlow @WIBRFlow]

Curious about cell cycle using #flowcytometry but not sure where to start? Start the New Year off right with @CrickTraining @FlowMskcc and WIBR Flow for our first OpenFlow class of 2022.

Free to attend and interaction is encouraged!

Register here: https://t.co/ECRUJbaM9D

[WIBRFlow @WIBRFlow]

Did you miss our latest #OpenFlow #flowcytometry class where we discussed panel design considerations with @CrickTraining & @FlowMskcc ? Don't FRET! (Not the fluorescence resonance energy transfer kind...)

You can check out the recording here: https://t.co/TOL2uZByF0

[WIBRFlow @WIBRFlow]

@AlvaroQuintanal @charlesrudin I see some wonderful people in this photo!

[WIBRFlow @WIBRFlow]

Don't forget! This Friday along with @CrickTraining & @FlowMskcc we will be giving a free course on panel design, incorporating both theory and practice through data analysis! Free and open to all.

[WIBRFlow @WIBRFlow]

Join @CrickTraining @FlowMskcc and us next week on 11/19 as we continue our discussion about panel design for flow cytometry! Free and open to all. Engagement from participants is always encouraged.

Register here: https://t.co/eFlm2KW8w0

[WIBRFlow @WIBRFlow]

@CrickTraining @RoyalMicroSoc Congratulations!!!!!!!

[flowMSKCC @FlowMskcc]

#FlowPostIt Curious how to test performance of your instrumentation in order to detect small particles? @WIBRFlow and us have some useful information on how to tackle these experiments! 👇 #flowcytometry #smallparticles #flowedu #flowcyto