AN

Political account. Everything I say reflects my own views only.

[AN @AliNehm01126348]

وَأُفَوِّضُ أَمْرِي إِلَى اللَّهِ

I entrust my affair to God

[Neal Amin @neal_amin]

Today, Sculpta releases the first demonstration of bioorthogonal barcoding ("bobcoding"), the process by which we:

1) covalently attach oligonucleotide barcodes to RNA at multiple internal positions with two-step chemistry, and 2) perform a multiplexed reverse transcription reaction to generate barcoded cDNA libraries with >99% barcoding accuracy.

To the best of our knowledge, it is the first time ever that either of these steps have ever been performed by anyone. No one has ever broken the barrier of multiplexing prior to any enzymatic step in library preparation, driving simplicity and higher data quality. Instead of other methods that add 0 or 1 barcode, we add one barcode every ~300bp, adding redundancy and fidelity to RNA measurements.

In our first application described in our pre-print below, we used bobcodes to create the first RNA isoform-resolved drug screening platform. It beats Novartis' DRUG-seq platform by:

- generating full length transcript capture of RNA isoforms, instead of just 3' end counting
- capturing 25-fold more RNA splicing events
- reducing barcode swapping by 10-fold
- using 18 fewer PCR cycles (~250,000 less amplification)
- reducing sample-to-sample variability in gene expression measurements
- eliminating costly and cumbersome library fragmentation/tagmentation steps completely
- reducing workflow complexity and number of steps
- reducing overall protocol duration by ~25%

Though our chemical barcoding method improved transcriptomic data quality while also being simpler and faster than all existing methods, the implications of this work go well beyond this particular use case:

The gate to greater applications of AI in transcriptomics is not a lack of compute. Nor is it a lack of data volume. The elephant in the room has always been our limited ability to faithfully and accurately measure cellular RNAs.

Sculpta now has line of sight to build what we call the first ground truth transcriptomics platform [for single cell, spatial and more] for the future of biological research, drug development and AI-enabled discoveries for the betterment of human health and longevity.

[PS Until the kind folks at bioRxiv get through the apparent backlog of AI slop submissions, Sculpta will host the pre-print PDF on our website. You can sign up for product offerings and other updates at this link too]

https://t.co/QPApERi7RT

[Abier @abierkhatib]

It’s time to normalize the phrase “Jewish terrorists” when discussing Jewish-violence against Palestinians. Consistency matters, if “Islamic terrorists” is acceptable language, so is “Jewish terrorists”

[Censored Humans @CensoredXHumans]

CNN will never show you this : An Israeli settler pointing a gun at a Palestinian farmer.

"This land is mine now. Get out. Never come back"

The farmer's family worked that land for generations.

The settler flew in from Eastern Europe.

[علي @allouush]

جوزيف عون كلّم.. الزلمي عم يمسح الأرض فيك كلّ أسبوع.

🤣🤣😭

[Israel Exposed @xIsraelExposedx]

It took a little longer than expected, but we have created a website for people to view the footage collected from Gaza in one place. You no longer have to download the entire archives to see them.

It includes:

64,537 videos
17,905 photos
Ability to download individual videos
Searchable index
Exhaustive sources list (300+ journalists)
Geolocation data
Livemap with minute to minute updates
Victim list

It can be accessed here: https://t.co/s0Se94PXWF

Please share & quote tweet to help this post break out of the twitter algorithm prison.

We will keep adding the rest of the archives to the site, be patient- it is difficult work. Continue to seed the torrents provided, as that is the best way to ensure the footage remains stored in decentalized way.

God bless all those who sacrificed their lives to get this footage out, and everyone invovled in collecting/archiving it.

Join our telegram:
https://t.co/bvcis3b9GT
Follow our backup accounts:
@ZionismExposedx & @IsraelExposedAr

[hala Haddad 10452 Km2 @halahaddad8]

_خلونا نجرب الدبلوماسية..

_خلونا نجرب التفاوض المباشر...

_خلونا نجرب مع المكانيزم يمكن نصلح الأسطل...

_خلونا نجرب تطبيق اتفاق الإطار....

حسي حالي فأر تجارب بمختبر اسمه لبنان...

حبيبي القضايا السيادة والوطنية مش نظام ريجيم منجربو يمكن نضعف، يمكن لا....

[George Galloway @georgegalloway]

He is SIX years old…

[Kamal khalaf @Kamalkhalaf17]

هذا هو ملخص الاتفاق بالصوت والصورة .. نتنياهو : سنبقى في المناطق الحيوية لنا في جنوب لبنان وفق الاتفاق ، حاول الايرانيون اخراجنا بكل الوسائل ولكن حققنا انجاز عظيم .. نشكر حكومة لبنان .

[ابراهيم ناجي Ibrahim Naji @Ibrahim___Yemen]

We did not see the Lebanese army defending Lebanon, but rather we saw them suppressing the Lebanese people who rejected the surrender agreement to America and Israel.
This is not a Lebanese army, but an Israeli security apparatus.

[Gaza Notifications @gazanotice]

⚠️ Extremely sensitive content⚠️

🚨A Palestinian child suffered a devastating head injury in an Israeli airstrike in Gaza. Despite severe trauma and extremely limited medical resources, doctors managed to save his life.

As documented by Dr. Jamal Salha, the child underwent emergency treatment after brain tissue had protruded from his skull. With operating rooms overwhelmed by casualties, the procedure was performed in the emergency room an extraordinary effort that saved him from death.

[Jvnior @Jvnior]

This is how Israelis settle.

They enter Palestinian homes.

Then they call the IDF to protect them.

It’s time to put an end to these war crimes.

[Hassan Mafi ‏ @thatdayin1992]

This is what terrorists look like

[جعفر فضل الله @JaafarFadlallah]

#رُوي
أن الدولة اللبنانية وقعت على اتفاق يجعل لمحتل أرضها منع مواطنيها من الرجوع إلى أراضيهم، وتجريم مواطنيها إذا عملوا على تحريرها من محتلّها..
رواية لا يستطيع حتى إبليس أن يصوغها!
كل هذا لإنقاذ مجرم الإبادة الأكبر من السقوط داخليًا!!!
عندما تلبس الخيانة ثوب السيادة!

[AN @AliNehm01126348]

@LBpresidency مصدق حالك صرت رئيس

[AN @AliNehm01126348]

@LBpresidency هيدا مش تدخل؟ سألوكم؟

[Earth Hippy 🌎🕊️💚 @hippyygoat]

STOLE THEIR KNEES
&
Stole their homes.
Stole their land.
Stole their livelihoods.
Stole their schools.
Stole their hospitals.
Stole their dignity.
Stole their freedom.
Stole their futures.
Stole their childhoods.
Stole their loved ones.
Stole their peace.

[Abdulsamet Altaş @SametAlta10]

BENDEN NE OLUR DEME…

"Bu videoyu izleyen ve bugüne kadar Filistin hakkında tek bir paylaşım yapmamış olan her sayfa sahiplerine sesleniyorum:
Bu videoyu geçebilirsin, ama bu akşam kafanı yastığa koyduğunda o çocukların çığlığından kaçamayacaksın. Şimdi bir hikaye paylaş ve sessizliğini boz."☝🏼🗣️🇵🇸

[Palestine Daily News✌️🇵🇸✌️ @Palestinedaily1]

⭕️Children around the world are safe, while #children_of_Gaza_are_being_killed every day