Anna Delgado Tejedor

Final year PhD Student at Epitranscriptomics and RNA Dynamics Lab @CRGGenomica @NovoaLab

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat @rodalies - fareu alguna cosa? És el pic de calor, un tren amb molta gent i sense aire...

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat Estem en el tren vintage direcció a Blanes (amb retard) sense aire el día més calurós de l'any... El vostre servei és impresentable.

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat @rodalies @rod1cat Portem més de 10 minuts aturats a Badalona, direcció el Hospitalet. Podeu informar sobre la incidencia?

[Anna Delgado Tejedor @AnnaDelgadoT]

@rodalies @LaiaCabellos @rod1cat Per Llavaneres han 'desaparegut' tres trens com a mínim en 45 minuts... Deixeu-vos d'eufemismes i informeu.

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat Entre trens que desapareixen i la resta que va tard... Sense informar, no sigui cas que féssiu la vostra feina de tant en quant. No teniu vergonya.

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat @rodalies podeu informar?

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat Per què només pasen trens curts (tot el sentit a l'estiu eh) cada 15min amb final a Mataró per St.Adrià? I els que arriben, impossible de pujar perquè van a petar... Vergonya us hauria de fer aquest servei lamentable.

[Anna Delgado Tejedor @AnnaDelgadoT]

https://t.co/mw3aCySCQo

[Novoa Lab @NovoaLab]

Check out our latest preprint!
m6A basecalling from direct RNA nanopore data at single nucleotide and single molecule resolution!!!

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat @govern @rodalies Anem com anoxes en el tren direcció Calella (curt!)! Podrieu posar trens llargs i no només acumular retrassos? La freqüència ja es queda curta de normal, a l'estiu és totalment insuficient. Vergonya us hauria de fer 😡

[Luca Cozzuto @lucacozzuto]

Today is the last day of the ROPES summer school at @CRGenomica. @AnnaDelgadoT from @NovoaLab is introducing Master of Pores 2: a suite for analysing nanopore data. A collaboration with @biocorecrg

[Anna Delgado Tejedor @AnnaDelgadoT]

@rod1cat Estem parats fa minuts a Vilassar... Quina normalitat es aquesta? Almenys podrieu informar...

[Novoa Lab @NovoaLab]

Want to hear about our new method Nano-tRNAseq published recently in @NatureBiotech? @MorghanLucas will present it online at the next @EUSynBioS seminar on April 27th at 16:00 CET 🙌 Register through the QR code or here: https://t.co/fsYXRZP6Lz

[Anna Delgado Tejedor @AnnaDelgadoT]

Thanks to co-authors @RebecaenTandem, @oguzhanbegik, @lucacozzuto, Julia Ponomarenko and @EvaMariaNovoa. As well as @NovoaLab and @CRGenomica.

[Anna Delgado Tejedor @AnnaDelgadoT]

Happy to share our preprint on using native RNA nanopore sequencing to reveal antibiotic-induced loss of rRNA modifications. https://t.co/DoqODA19at 🧵
#nanopore #antibiotics #epitranscriptome

[Anna Delgado Tejedor @AnnaDelgadoT]

Our work reveals that rRNA modification profiles can be rapidly altered in response to environmental exposures, and that nanopore sequencing can accurately identify dysregulated rRNA modifications, contributing to the mechanistic dissection of antibiotic resistance. 🦠💊

[Anna Delgado Tejedor @AnnaDelgadoT]

We then systematically examined whether loss of "antibiotic-sensitive" rRNA modifications may be sufficient to confer antibiotic resistance, finding that depletion of some rRNA modification enzymes guiding dysregulated rRNA modifications confers increased antibiotic resistance.

[Anna Delgado Tejedor @AnnaDelgadoT]

We found that significantly altered rRNA modified sites upon antibiotic exposure are located in the vicinity of the A and P-sites of the ribosome, possibly contributing to antibiotic resistance.

[Anna Delgado Tejedor @AnnaDelgadoT]

NanoConsensus is robust across RNA modification types, stoichiometries and coverage, and outperforms all individual algorithms tested. We identified multiple rRNA modifications that are lost upon the presence of antibiotics in an antibiotic-specific manner.

[Anna Delgado Tejedor @AnnaDelgadoT]

To identify rRNA modification changes, we developed NanoConsensus, a pipeline that integrates the estimates from multiple RNA modification detection algorithms, predicting differentially modified rRNA sites with very low false positive rates and high replicability.