Nolan Amon

Ph.D student in entomology, @UWMadison. Studying microbial ecology of wild bees. Hate wearing shoes. He/him/his

[Nolan Amon @Anthophilist]

My first, first author paper got published :) https://t.co/j8fhJNxT1p

[Nolan Amon @Anthophilist]

Hello Science Twitter! I do still exist, and am doing lots of fun metagenomics things. On that note, I was wondering how I might be able to retrieve the entire taxonomic hierarchy for a list (~2000) of organisms. There seems to be an R package (taxize) but it seems broken :(

[Nolan Amon @Anthophilist]

(why yes I did just get my first illumina seqs back and now I'm messing around like a toddler who got into power tools, why do you ask?)

[Nolan Amon @Anthophilist]

Hey science Twitter, it's been awhile!! So just wondering, what are everyone's favorite programs for classification in shotgun metagenomics and if you're using kraken2 what is your favorite database and confidence score to use? 👉👈

[Nolan Amon @Anthophilist]

I've been fiddling with some R code for three days and it FINALLY is working, there's almost nothing more gratifying.

[Nolan Amon @Anthophilist]

My son, the bee

[Nolan Amon @Anthophilist]

I was chosen 😤

[Nolan Amon @Anthophilist]

Trying to figure out coverage based rarefaction with #iNext in R- lots of examples given where you are comparing two groups/treatments with a single collection event, but I can't wrap my brain around how to handle data sourced from multiple collection events! Any advice?

[Nolan Amon @Anthophilist]

At the barber today and he asked me if I go to school in Madison. I said yes, and then he followed up by asking what grade I was in- as he was cutting my head full of graying, Ph.D. related hair 😅

[Nolan Amon @Anthophilist]

@Jenna_M_Walters I agree with Bombus- I think this is a very weird, very sad impatiens!

[Nolan Amon @Anthophilist]

Please tag any aquatic ecologists who might know!

[Nolan Amon @Anthophilist]

Culturing some pond water to try getting live food for my fish, does anyone know what these y-shaped/tri-lobed little guys are?

[Nolan Amon @Anthophilist]

It took me literally all day and about 100 different browser tabs but I analyzed my first non-tutorial microbiome dataset :)

[Nolan Amon @Anthophilist]

@tahneeames I'll let you know once the samples come back ;)

[Nolan Amon @Anthophilist]

Samples dropped off this morning! Can we get good enough quality and quantity for metatrancriptomics? I'm doubtful but time will tell!

[Nolan Amon @Anthophilist]

@tahneeames The samples are in RNAlater! But our biocore set out a pail of wet ice for sample submission, even RNA 🤷‍♂️

[Nolan Amon @Anthophilist]

Running an ordination on some community data- I don't know what Procrustes means and at this point I'm too afraid to ask.

[Nolan Amon @Anthophilist]

How would one hypothetically begin to organize a general strike

[Nolan Amon @Anthophilist]

For reference, community is relatively species rich (119 species) but not very even- almost a quarter of those 119 species are represented from only 1 or 2 collection events. Strong bias towards halictids, as might be expected from pan traps.

[Nolan Amon @Anthophilist]

Hello nerds! Working on stats for my master's research examining effects of wildflower plantings on wild bee communities in cranberry. Which biodiversity metrics/indices do you think should be reported in a manuscript? (1/2)