Atefeh NamiPashaki

Resarch Fellow @unimelb

[bioRxiv Neuroscience @biorxiv_neursci]

Transcriptome profiling of dopaminergic neurons derived from an ADHD induced pluripotent stem cell (iPSC) model https://t.co/tS42W9UK5G #biorxiv_neursci

[Atefeh NamiPashaki @AtefehNami]

@DaniBeckman Wow! Beautiful!

[Atefeh NamiPashaki @AtefehNami]

@kealanpugsley Thanks to you 🙏😊

[Atefeh NamiPashaki @AtefehNami]

It’s out.
If you are struggling to CRISPR your pluripotent stem cells (PSCs), especially those who are working in the field of iPSC disease modeling in the post-GWAS era, you can consider this method.

Full article: https://t.co/7vPe6NZtbf

[ESC & iPSC News @ESC_iPSC_News]

Definitely a worthy methodology for those in the field to check out. Congratulations to the team! 👏

[Atefeh NamiPashaki @AtefehNami]

@TraceyNeuro @BellgroveMark Congratulations Tracey!!

[Atefeh NamiPashaki @AtefehNami]

Still a long way to high-throughput CRISPRing iPSCs, but this can be a moving forward step.
Hoping this can benefit the field in characterizing the functional importance of risk SNPs underlying disease etiology.

[Atefeh NamiPashaki @AtefehNami]

This is an intensively optimized method that benefits from the integration of RNP lipofection into single-cell cloning of CRISPRed iPSCs,achieving a homogeneous knocked-in/out iPSC line in a short time-No more successive rounds of ddPCR or mixed heterogenous antibiotic selection!

[Atefeh NamiPashaki @AtefehNami]

@huwbris @PNASNews @trevor_chong Congrats Huw, Amazing work!

[Atefeh NamiPashaki @AtefehNami]

@rachael_knott1 Congrats Rachel. Amazing work!

[Atefeh NamiPashaki @AtefehNami]

@rachael_knott1 Thanks Rachael ☺️

[Atefeh NamiPashaki @AtefehNami]

@TraceyNeuro Thanks Tracey ☺️

[Atefeh NamiPashaki @AtefehNami]

Our ADHD patient-derived dopaminergic neurons bursts in response to depolarisation dependant Calcium influx!
So cool to watch! :))

#FluorescenceFriday

[Atefeh NamiPashaki @AtefehNami]

@huwbris @DementiaAus @_IsabelleBurke Congrats Huw! Very well done 👍

[Atefeh NamiPashaki @AtefehNami]

@KittelSchneider @BellgroveMark @kealanpugsley 👍

[Atefeh NamiPashaki @AtefehNami]

@KittelSchneider @BellgroveMark @kealanpugsley Dear Sarah, Thank you very much for sharing your amazing paper. you are right, with the difference of these being induction based highly homogenous.

[Atefeh NamiPashaki @AtefehNami]

@smwrajaratnam @BellgroveMark Thank you very much Shantha

[Atefeh NamiPashaki @AtefehNami]

@kealanpugsley Thanks Kealan for all your support! 🥰

[Atefeh NamiPashaki @AtefehNami]

@huwbris @BellgroveMark @kealanpugsley Thanks Huw!☺️