Any #microscopy friends have any suggestions for a water stage cooler/pump? I'm specifically interested in something to slowly flow water through a light sheet imaging chamber so it will need a very slow and consistent flow rate. Maybe @ZakSwartz ?
How does RGA get recruited to cortical waves and what exactly is the nature of its relationship with F-actin? We aren't sure, but we plan to use immature frog oocytes and modeling to address these questions!
Our latest pre-print is up on @bioRxiv! Here, @AniMichaud1 et al. identified Ect2 and RGA-3/4 as core components of cytokinetic cortical excitability. We also show that immature Xenopus oocytes are a powerful model for characterizing excitability. A 🧵...
https://t.co/NE8lIxLov1
Modeling and experiments showed that Ect2 and RGA-3/4 are major drivers of this circuit, and just altering the relative levels of these two players produced a variety of cortical behaviors in an otherwise quiescent oocyte cortex!