BioProcess Tools

Free tools for fermentation & cell culture scientists
Replace your scattered Excel sheets with calculators and trackers built by bioprocess engineers.

[BioProcess Tools @Bioprocesstools]

Cell Seeding Density: How Many Cells to Plate & Split Ratio, in one short video.

0.3 × 10⁶ cells/mL in a 15 mL T75 = 4.5 × 10⁶ cells: 0.75 mL of a 2 × 10⁶/mL culture + 14.25 mL medium.

Free calculator: https://t.co/Kkd6Rz0Iqs

[BioProcess Tools @Bioprocesstools]

Methanol-free Pichia now beats methanol-induced: 3.7 vs 3.1 g/L for nanobodies, no ATEX facility needed, 48h vs 96h runs. Full promoter guide: https://t.co/wBD4VYGQKu #Bioprocessing #PichiaPasstoris

[BioProcess Tools @Bioprocesstools]

A NIR protein analyzer claiming R² = 0.9998 with no PLS model. Published NIR-bioprocess work tops out near 0.97-0.99.

Zero peer-reviewed deployment papers yet. How to evaluate it anyway:

https://t.co/rknC6U4wR8 #Bioprocessing

[BioProcess Tools @Bioprocesstools]

IPTG Induction: IPTG Concentration, Induction OD600 & Temperature, in one short video.

Typical 0.1-1 mM; LacI saturates ~0.1-0.3 mM. Induce at OD600 0.4-0.8. First try: 0.1 mM, 18 °C overnight.

Free optimizer: https://t.co/yacu8smRyC

[BioProcess Tools @Bioprocesstools]

Hemocytometer Calculation: Cells per mL, Dilution Factor & Viability, in one short video.

cells/mL = avg per square × dilution factor × 10⁴. 240 cells in 4 squares, 1:1 trypan blue: 1.2 × 10⁶ cells/mL.

Free calculator: https://t.co/QjHTiZHreR

[BioProcess Tools @Bioprocesstools]

N-1 perfusion vs concentrated fed-batch vs frozen bag: which one fits YOUR facility?

Head-to-head COGS data + decision framework:

https://t.co/mDwiQooT21

#ProcessIntensification #Biologics

[BioProcess Tools @Bioprocesstools]

Four biomanufacturing decarbonisation projects. Their standalone savings add up to 147.7%. Run together, the cut is 92%.

The levers multiply, so the order decides what each one is worth.

https://t.co/YEGq0Y3MYJ #Bioprocessing

[BioProcess Tools @Bioprocesstools]

RPM to RCF: How to Convert RPM to g-Force and RCF to RPM, in one short video.

RCF = 1.118e-5 × r (cm) × RPM². r runs from the axis to the tube bottom. 300 ×g on a 17.5 cm rotor ≈ 1,238 RPM.

Free RPM to RCF calculator: https://t.co/G1axy28lVs

[BioProcess Tools @Bioprocesstools]

Why does your CHO feed precipitate after a week? Tyrosine: 0.45 g/L solubility, you need 1.6 g/L. Phospho-tyrosine fixes it at 70 g/L, pH 7.

Full feed concentrate design workflow here:
https://t.co/sCXKwjAp98

#CHOCellCulture #Bioprocess

[BioProcess Tools @Bioprocesstools]

E. coli: gene to protein in 1-2 weeks. Pichia: 4-8 weeks. A clonal CHO cell bank: 7-14 months.

All three land near $50/g at scale. Pick the cost you can absorb, not the titer.

https://t.co/ImwNPtrFbZ #Bioprocessing

[BioProcess Tools @Bioprocesstools]

Tm Calculator: How to Calculate Primer Tm and Annealing Temperature, in one short video.

Wallace: 2(A+T) + 4(G+C). Nearest-neighbour: typically ±1-2 °C. Anneal ~5 °C below the lower Tm.

Free Tm calculator: https://t.co/IeFAu6u1rT

[BioProcess Tools @Bioprocesstools]

How do you design a DO cascade that works at 2 L AND 2,000 L? Agitation, gas flow, O2 enrichment: the order matters, and each level needs its own PID tuning.

https://t.co/FhNGU2R14C

#Bioprocess #DOControl

[BioProcess Tools @Bioprocesstools]

Two CHO runs, same 20.1e6 cells/mL peak, same 14 days. One has 14% less IVC, about 0.7 g/L of titer, all lost after the peak.

Titer = qP x IVC. Compare runs on IVC, not peak VCD.

Free IVC + qP calculator:
https://t.co/wCrHwV89cq #Bioprocessing

[BioProcess Tools @Bioprocesstools]

kLa explained: what it measures, why OTR = kLa × (C* − C), and the 3 levers that raise it.

Mammalian cells: ~5–20 h⁻¹. High-density microbial: several hundred.

Free estimator: https://t.co/z8jBNwid8d

[BioProcess Tools @Bioprocesstools]

Why does precision fermentation stall between 1,000 and 50,000 L? At 100 g/L biomass, OUR hits 150-300 mmol/L/h. That's 30-100x more than CHO. Full scale-up engineering guide:

https://t.co/8o8nQdkCJL

#PrecisionFermentation #ScaleUp

[BioProcess Tools @Bioprocesstools]

Constant P/V and constant kLa give nearly the same rpm at scale. Until the gas changes: halve superficial gas velocity and kLa drops ~30% at identical P/V.

https://t.co/CGJsO4xbu8

#Bioprocessing

[BioProcess Tools @Bioprocesstools]

How many DOE runs does a biologics process characterization study need? 40-80 across screening + optimization. Full guide with FMEA, PAR/NOR, and design space:

https://t.co/JJ4mjIeMBZ

#ProcessCharacterization #QbD

[BioProcess Tools @Bioprocesstools]

Same 2,000 L of CIP rinse water: one full rinse removes 2.3 logs of residue, ten 200 L rinses remove 13.2. Dilution is geometric. 6x less water for the same validated 6-log clean.

https://t.co/u4u7wcnf94

#Bioprocessing

[BioProcess Tools @Bioprocesstools]

Acoustic separation: >98% cell viability, no membranes, no centrifuge. Force scales with R³ so mammalian cells separate while bacteria don't.

https://t.co/wA0t0zCgr6

#Bioprocessing

[BioProcess Tools @Bioprocesstools]

Optical pH spots read 0.072 pH off vs 0.044 for glass. The bigger issue: they cluster near setpoint and lose accuracy past +/-0.25 pH. The drift you need to catch is the one they hide.

https://t.co/6hobiokLTC #Bioprocessing