Dan Lin

Research scientist @DanforthCenter; studying plant-microbe interactions. Current emphasis on cassava & viruses. Views are my own.

[Barack Obama @BarackObama]

44 days.

That’s all the time we have left until Election Day.

@KamalaHarris has the vision, strength, and compassion to lead this country forward. Make your voice heard—make a plan to vote early, vote by mail, or head to the polls. https://t.co/bBcatjmEym

[Dan Lin @DLinYZ]

@ChenxinLi2 Awesome, thanks for sharing!

[Dan Lin @DLinYZ]

@ChenxinLi2 Great images! Were the flashlights pretty easily obtainable? I found ones that work well with my favorite GFP, would be great for other FPs as well.

[Becky Bart @becky_bart]

Thanks to everyone @DanforthCenter and in the Bart and Carrington labs for a successful @PlantTechJam! What did we learn? Plants get sick and microbes are fun.

[Dan Lin @DLinYZ]

@BenMansfeld I agree re: not going cheap!

New Gilson's are easy on thumbs like Rainin LTS. Eppendorf's are nice, too. Problem with Rainin is being trapped in their tip ecosystem.

Definitely demo, and think about adjustable spacing multichannels to maximize throughput!

[Dan Lin @DLinYZ]

@klsywd @albalmuth 3xFLAG is probably my favorite tag, but HA or 3xHA should work for IPs, too!

[Dan Lin @DLinYZ]

@aplantbiologist That's what my team has experienced. Worked with GV3101 lots before LBA4404 and was surprised by the latter's clumping behavior.

[Dan Lin @DLinYZ]

@ChenxinLi2 I had a rude awakening when I realized my TuMV-GFP and eGFP constructs didn't work equally well under 390 nm UV lamp. Turns out viral GFP label excites at 390 nm while eGFP excites at 488 nm. I now check all my FP sequences on https://t.co/hv9mdRMjUK

[Dan Lin @DLinYZ]

@ChenxinLi2 There are a lot of GFP variants with slightly different excitation/emission spectra. Are the excitation wavelength settings the same for 10-20x scope, dissecting scope, and flashlight?

[Dan Lin @DLinYZ]

@LizahvdAart This is amazing, Lizah! I grappled with this all the time when I was in grad school but have come to the same conclusions. Thanks for sharing!

[Dan Lin @DLinYZ]

@roszenil @JenniferMandel In this boat! I don't have a green thumb or an encyclopedic knowledge of house plants.

Friends: "Hey, Dan, what's this plant?"
Me: "Uhhh... it's not arabidopsis, that's for sure!"

[Dan Lin @DLinYZ]

Bart Lab is looking for a post doc to study the plant microbiome as part of @DanforthCenter 's SINC initiative https://t.co/e704bfzkGK. Come join us! @becky_bart is a great mentor and person, and we're a tight-knit group of collaboration minded individuals!

[Becky Bart @becky_bart]

Plant microbiome postdoc position available in the Bart lab. https://t.co/QjV4Ja13yU

[Dan Lin @DLinYZ]

I love the associated picture and story. Great lesson on survivorship/selection bias.

https://t.co/X7hulepu2K

[John McEwan @mcewanjc]

@Grey_Monroe @Nature What is called the "mutation rate" is estimated *after* various repair mechanisms have operated (which are in themselves heritable) and the individual has survived to a level where it can be genotyped. Better to say that the rate is also affected by the epigenome.

[Dan Lin @DLinYZ]

@PlantPathSecret I've been in two labs where this happened (not my fault!), both with touch-o-matic type burners. First time, building safety officer banned gas lines into clean hoods, and we ended up switching to cooking torches!

[Dr. Nadia Chaudhri @DrNadiaChaudhri]

Now that I have 100K followers, I want to talk about #OvarianCancer. Specifically my gritty story. The goal is awareness. I hope you find this narrative informative.

[Dan Lin @DLinYZ]

@ChenxinLi2 Great question. Looks like in lieu of including a clamp, polymerases in PCR can be fused to sequence non-specific DNA binding proteins:

https://t.co/fZiGrYnMIo

+ clamp seems to be orders magnitude better processivity, tho

[Dan Lin @DLinYZ]

@pollenmicrobes @a_gneiss_birb Awww, this makes me super sad – my daily commute for 6 years involved riding beneath those beautiful trees.

[Dan Lin @DLinYZ]

@byu_sam Years ago at Berkeley I was bamboozled into majoring in "Genetics and Plant Biology", not realizing what I actually wanted at the time was MCB w/ emphasis on genetics.

Happily still studying plant biology many years later, though!

[ACLU @ACLU]

George Floyd will never again play games with his daughter, Gianna.

He’ll never go on walks through the park with his fiancée, Courteney.

He will never play basketball with his brother, Philonise.

True justice would mean George Floyd was never killed in the first place.