44 days.
That’s all the time we have left until Election Day.
@KamalaHarris has the vision, strength, and compassion to lead this country forward. Make your voice heard—make a plan to vote early, vote by mail, or head to the polls. https://t.co/bBcatjmEym
@ChenxinLi2 Great images! Were the flashlights pretty easily obtainable? I found ones that work well with my favorite GFP, would be great for other FPs as well.
Thanks to everyone @DanforthCenter and in the Bart and Carrington labs for a successful @PlantTechJam! What did we learn? Plants get sick and microbes are fun.
@BenMansfeld I agree re: not going cheap!
New Gilson's are easy on thumbs like Rainin LTS. Eppendorf's are nice, too. Problem with Rainin is being trapped in their tip ecosystem.
Definitely demo, and think about adjustable spacing multichannels to maximize throughput!
@ChenxinLi2 I had a rude awakening when I realized my TuMV-GFP and eGFP constructs didn't work equally well under 390 nm UV lamp. Turns out viral GFP label excites at 390 nm while eGFP excites at 488 nm. I now check all my FP sequences on https://t.co/hv9mdRMjUK
@ChenxinLi2 There are a lot of GFP variants with slightly different excitation/emission spectra. Are the excitation wavelength settings the same for 10-20x scope, dissecting scope, and flashlight?
@LizahvdAart This is amazing, Lizah! I grappled with this all the time when I was in grad school but have come to the same conclusions. Thanks for sharing!
@roszenil @JenniferMandel In this boat! I don't have a green thumb or an encyclopedic knowledge of house plants.
Friends: "Hey, Dan, what's this plant?"
Me: "Uhhh... it's not arabidopsis, that's for sure!"
Bart Lab is looking for a post doc to study the plant microbiome as part of @DanforthCenter 's SINC initiative https://t.co/e704bfzkGK. Come join us! @becky_bart is a great mentor and person, and we're a tight-knit group of collaboration minded individuals!
@Grey_Monroe@Nature What is called the "mutation rate" is estimated *after* various repair mechanisms have operated (which are in themselves heritable) and the individual has survived to a level where it can be genotyped. Better to say that the rate is also affected by the epigenome.
@PlantPathSecret I've been in two labs where this happened (not my fault!), both with touch-o-matic type burners. First time, building safety officer banned gas lines into clean hoods, and we ended up switching to cooking torches!
Now that I have 100K followers, I want to talk about #OvarianCancer. Specifically my gritty story. The goal is awareness. I hope you find this narrative informative.
@ChenxinLi2 Great question. Looks like in lieu of including a clamp, polymerases in PCR can be fused to sequence non-specific DNA binding proteins:
https://t.co/fZiGrYnMIo
+ clamp seems to be orders magnitude better processivity, tho
@byu_sam Years ago at Berkeley I was bamboozled into majoring in "Genetics and Plant Biology", not realizing what I actually wanted at the time was MCB w/ emphasis on genetics.
Happily still studying plant biology many years later, though!
George Floyd will never again play games with his daughter, Gianna.
He’ll never go on walks through the park with his fiancée, Courteney.
He will never play basketball with his brother, Philonise.
True justice would mean George Floyd was never killed in the first place.