@pseudotsugonoid fwiw - I never heard about the GG issue you mentioned. I just spot checked some samples from a novaseq 2x250 single 12bp indexed run and there were plenty of reads for samples with GG on either end of the index.
@mixotrophe Maybe you could modify the one on the left so the values are shown by the proportion of the box filled in as opposed to the hue/shade. That way you can keep the color scheme but the values would be easier to compare (and interpret).
@e_hannula @ldereske @MAnthony02 @PacBio ITSx extracts the synthetic sequences from the amptk paper just fine in my experience. The ITSx model only searches for the conserved regions flanking ITS which are based on real fungal sequences in the amptk synmock seqs.
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@BentonRochester@drewmck The relative abundances we recover from eDNA sequencing are subject to multiple sources of bias, and should be interpreted with that in mind. But your bees were most definitely consuming everything we detected.
@jdierks@BentonRochester This was done by sequencing plant DNA present in the honey, which is one of the eDNA sequencing services we offer @JonahVentures.
Devin Leopold is our bioinformatician. All the data you get back from us, whether in files or on the data portal goes through him first. Welcome to @JonahVentures, Devin!
HIPR-FISH: Highly multiplexed spatial mapping of microbial communities. Visualize microbes in the gut with high taxonomic resolution! It was super fun to collaborate with @DeVlaminckLab and @haoiseetheworld on this! @nature@CornellBME
I’m excited to invite applications for two PhD positions in my lab at UT Arlington! Love insects? Love the microbiome? Check it out and spread the word: https://t.co/1Q19NHBCEu
hi all! Im looking for a new postdoc on an NSF funded fungal endosymbiont interaction genomics project, see more detail at https://t.co/e0V1ltIbWE please RT