Edsinger Lab

Cephalopod research at the Marine Biological Laboratory

[Edsinger Lab @EdsingerLab]

Someone stole our female squid!

And swapped in a male.... unfortunately two males do not make for making squid babies!

On Friday we set up two large males and two small females in our holding tank for the weekend. On Saturday Atreyo did a fertilization… https://t.co/PE1dHH22ea

[Edsinger Lab @EdsingerLab]

Someone stole our female squid!

And swapped in a male.... unfortunately two males do not make for making squid babies!

On Friday we set up two large males and two small females in our holding tank for the weekend. On Saturday Atreyo did a fertilization… https://t.co/PE1dHH22ea

[Edsinger Lab @EdsingerLab]

12 hours to prep a sample and start collecting a developmental time lapse of a now 36 hour MBL squid embryo on the diSPIM light sheet microscope.
You can see how the two imaging arms collect images at -90 degrees to each other.

I’m moving slow and had … https://t.co/hCz1qWUMbd

[Edsinger Lab @EdsingerLab]

We got 4 new octopus yesterday - for an arm regeneration project that Kirsten Edepli is starting this summer.

This one pooped - not sure if it was shooting it me...! https://t.co/tH2J8ku3kj

[Edsinger Lab @EdsingerLab]

I’m moving slow but getting better at running and even writing FIJI macros for processing diSPIM light sheet microscope data.

Here is the same data set from before of a 2 day old squid embryo with fluorescent dye labeling the nuclei - but now the two op… https://t.co/8QO1Wj1VYJ

[Edsinger Lab @EdsingerLab]

I got basic diSPIM image processing worked out using FIJI - and generated a simple time lapse from the 15 hour squid embryo data set from Thursday!

The dye is fading from cell divisions and not sure if it’s laser or something else. Things will improve a… https://t.co/yO8chnSJFL

[Edsinger Lab @EdsingerLab]

This has been a great week for lab milestones!

After a month and a half of YouTube training, Kirsten is running the diSPIM and we are getting our first developmental time lapse data sets.

As of an hour ago Malaika has the minimal number of replicates n… https://t.co/J7fyPC8dn4

[Edsinger Lab @EdsingerLab]

This has been a great week for lab milestones!

After a month and a half of YouTube training, Kirsten is running the diSPIM and we are getting our first developmental time lapse data sets.

As of an hour ago Malaika has the minimal number of replicates n… https://t.co/J7fyPC8dn4

[Edsinger Lab @EdsingerLab]

Kirsten, Hari, Harsh, and I did what may be the first ever developmental time lapse of a cephalopod embryo on a light sheet microscope on Wednesday.

It was exciting but we used a 40x objective and because of the high magnification could only image a sma… https://t.co/ty6Crdllnp

[Edsinger Lab @EdsingerLab]

Malaika continues her quest for Mnemiopsis ctenophores for single-cell sequencing by collaborators.
Tonight still zero - but she did find this gorgeous postlarval lobster in the plankton!

Eric https://t.co/oXJJl9vLXl

[Edsinger Lab @EdsingerLab]

Pygmy squid Idiosepius pygmaeus vs MBL squid Doryteuthis pealeii

Hatchlings from today - Malaika is breaking jet propulsion in both - and testing if we can predict from the results of one at what viscosity the other one should break jet propulsion- base… https://t.co/34kE2aK3Lw

[Edsinger Lab @EdsingerLab]

Pygmy squid Idiosepius pygmaeus vs MBL squid Doryteuthis pealeii

Hatchlings from today - Malaika is breaking jet propulsion in both - and testing if we can predict from the results of one at what viscosity the other one should break jet propulsion- base… https://t.co/34kE2aK3Lw

[Edsinger Lab @EdsingerLab]

The hydrogel paper with Dirk Albrecht and Kyra Burnett is out!

It includes (I think) the first publication of live light sheet imaging in cephalopod - using pygmy squid hatchlings immobilized in hydrogel and fluorescent dye!

https://t.co/Cl7mmyKe0j

E… https://t.co/8JiW70q1OY

[Edsinger Lab @EdsingerLab]

The hydrogel paper with Dirk Albrecht and Kyra Burnett is out!

It includes (I think) the first publication of live light sheet imaging in cephalopod - using pygmy squid hatchlings immobilized in hydrogel and fluorescent dye!

https://t.co/Cl7mmyKe0j

E… https://t.co/8JiW70q1OY

[Edsinger Lab @EdsingerLab]

OceanX is posting some of our videos on Twitter for #cephalopodweek !

Friendly octopus hatchling is up - and Peter’s imaging of squid hatchling brains - cleared and labeled with dyes - is in the works!

Our original videos are on Vimeo - … https://t.co/hVCGytUqU0

[Edsinger Lab @EdsingerLab]

OceanX is posting some of our videos on Twitter for #cephalopodweek !

Friendly octopus hatchling is up - and Peter’s imaging of squid hatchling brains - cleared and labeled with dyes - is in the works!

Our original videos are on Vimeo - … https://t.co/hVCGytUqU0

[Edsinger Lab @EdsingerLab]

I’m going on the job market for academic positions starting in August - OIST (where I did my previous postdoc) may be my first application!

If you know of any positions in the coming months, please let me know!

In the meantime I will be wrapping up pro… https://t.co/4stAzKyhE2

[Edsinger Lab @EdsingerLab]

Atreyo has a python computational background in astronomy image processing, among other things - now he is building off it - and working with my scripts as training - to identify and pull out promoter sequence from octopus for Elav, beta-actin, synatophy… https://t.co/NkE3TnBKXD

[Edsinger Lab @EdsingerLab]

Sealed in amphipods and octopus in the skinnies!

Eric https://t.co/iSRSC21nuj

[Edsinger Lab @EdsingerLab]

It’s been an exciting weekend for live imaging - but I’m ending the night / morning with a massive set back.

All the gDNA that Kirsten and I have been preparing the last month is completely degraded due to a mistake I made.

We had resuspended the DNA … https://t.co/jHwtDU6jz3