Eshariah Dyson

Graduate student. Chemist. Research assistant. Loves books and nature.

[tracy clayton jr the 3rd @brokeymcpoverty]

black people riot when their anger over being murdered and oppressed reaches a boiling point.

here are some fun reasons why white people have rioted:

[Environmental Science & Technology Journals @EnvSciTech]

Understanding the #nanoparticle deposition process is important for predicting the transport of nanoparticles in natural and engineered aquatic environments. Find out more here: https://t.co/bZeN2JYGlN

[Kaaathy @LKath_]

@ASBMB thank you for this unique opportunity to present my work and learn more about other projects. Thank you to @ninagoodey and @montclairstateu for providing the facilities, tools, and guiding the project. And thank you to all my fellow collaborators. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

Thank you @ASBMB for the opportunity to present my research and interact with other members of the scientific community. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB @LKath_ @edenmgallegos @SB21_droso @NourFatema4 Q6: (3) peroxidase activity can be used as a proxy to understand the potential of a site to self remediate.

[Eshariah Dyson @Eshariah1]

@ASBMB @LKath_ @edenmgallegos @SB21_droso @NourFatema4 Q6: (1) despite the presence of contaminates, our site posses the ability to self remediate. (2) the barren area of our site may be inhibited by the presence of the metal layer concentrated at the top of the soil. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A5: This measurable activity indicates that this site which looks to be totally inactive, is not as "dead" as it appears. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A5:The second thing that surprised us was a measurable peroxidase activity in the barren site. We have done previous enzymatic activity analysis of enzymes associated with nutrient cycling at this site. These enzyme activities were low or below detection limit. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A5: The metal concentration in this cross section was higher than the deeper cross sections of that area and higher than the adjacent vegetated area. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A5: We found two things that really surprised us. The first was that the barren strip of land at our site had a significantly higher metal concentration in the top (0-2 cm) cross section. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A3: My group and I had to hike through the woods, in the heat, on barely maintained paths. It was definitely a great bonding experience for all of us. #ASBMBenzymes @KruminsLab @ninagoodey

[Eshariah Dyson @Eshariah1]

@ASBMB A3: One of the most challenging parts of this project was collecting the soil samples from the site. This area is a densely populated forest and our research site is located several miles within the forest. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A2: (part 4) l-3,4 dihydroxyphenylalanine was used as the substrate for the peroxidase enzyme. A Michaelis–Menten curve was constructed to determine the best concentration of substrate to use for this analysis. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A2: (part 3) To determine the peroxidase activity, soil slurries were made for each cross section of each site. The slurries were added to a 96 well plate and monitored for absorbance at 460nm on a plate reader for 3 hours. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A2: (part 2)Soils were dried, ground into a fine powder, and refluxed at 94°C in a 1:1 nitric acid and hydrogen peroxide mixture. After reflux, samples were cooled, filtered, and diluted. Diluted samples were analyzed on an ICP-MS for their metal concentration. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A2: (part 1) To determine the metal concentration within the soil cross sections of each site we used inductively coupled plasma mass spectrometry (ICP-MS). #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@angelahopp @ASBMB @montclairstateu It really is. This site is both vegetated and contaminated. The barren strip of land is a strange anomaly at the site and it provides a great opportunity to learn about what it takes for a site to self remediate. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A1: Why peroxidase? This enzyme is a remediation enzyme that acts to mitigate the effects of oxidative stress caused by high concentration of metals in the soil. A measurable peroxidase activity indicates a soil that is actively working to restore itself #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB A1: We took core samples from the vegetated and the barren site. We looked at the metal concentration through depth and the peroxidase activity through depth for each site to see how they differed from one another. #ASBMBenzymes

[Eshariah Dyson @Eshariah1]

@ASBMB On section of the site, which we refer to as site 25, contains a vegetated are adjacent to a barren strip of land completely devoid of any plant life. #ASBMBenzymes