Florian Scharhauser

Marine Biologist, marine symbiosis, PhD student, nematode aficionado

[Jean-Marie Volland @JeanVolland]

My best friend Fabian, his wife Juliane, and their kids Eliora & Leander lost their home to the Eaton fire. They spent 2 years building their dream home, only to see it destroyed in minutes. 💔
🙏Let’s help them rebuild. Every share & donation counts: https://t.co/rCsayqvYud

[Florian Scharhauser @FScharhauser]

@TinkaraTinta Congratulations!

[Florian Scharhauser @FScharhauser]

@de_oliveira86 @bright_monika Congratulations!

[Florian Scharhauser @FScharhauser]

@Alex_Holovachov @SvenskaArtproje @naturhistoriska @nanopore Congratulations!

[Florian Scharhauser @FScharhauser]

@tendersombrero Cool, that you found them! Do you have a photo, maybe? Just curious.😊

[Florian Scharhauser @FScharhauser]

I am very happy to share with you the wonderful story of strange, dark dotted nematodes in the sea. Thank you to the whole team of co-authors, especially to @GruberVodicka and @NLeisch. https://t.co/JTkAltBBQM Please read more about these special members of the #Stilbonematinae

[Florian Scharhauser @FScharhauser]

@NLeisch @MAG_Porras Woah, what a crazy awesome story! Thank you for the lovely sum up.. Congratulations!

[Florian Scharhauser @FScharhauser]

@NicoleDubilier Congratulations, absolutely amazing!

[Florian Scharhauser @FScharhauser]

@NLeisch Nice! Is it a Catanema? ;-)

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 Thank you! Yes, very likely you lost a bit of skin. I always work with Gloves to keep contamination low.

[Max Planck Institute for Marine Microbiology @MarineMicrobio]

TV-Time: Kein Mund, kein Po, und trotzdem froh! 🦠🪱

Schöner Beitrag auf @3sat über die Forschung von @NicoleDubilier und unserer #Symbiose-Abteilung an darmlosen Würmern und ihren bakteriellen Bewohnern – ab Minute 13!

https://t.co/oCiOkO91gc

#Chemosynthese @maxplanckpress

[Nicole Dubilier @NicoleDubilier]

Seagrass meadows are among the most productive ecosystems in the world. Check out this documentary by Florian Guthknecht showing research by @MarineMicrobio @MarineEvolEcol @GEOMAR_en @O_LiviaRoth on seagrass.
https://t.co/NHBQfgvBAk

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 Ok, I would highly recommend to UV your tubes, pipette tips and your water before mixing. The primers can pick up funny things if they have the wrong concentration, I agree. But let's first rule out the decontamination issues.

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 Concentration could be 50 , 20 or 10. I usually go with 10 if there is enough DNA.

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 Ok, but the primers have to pick up something in this case. The primers alone can't produce a band as large and sharp as you have it in your gel. Do you UV your water and the PCR tubes you are using?

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 For negative controls?

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 Very good!. Let us now when you have the results.

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 You could also try to sequence the contamination/ the band in the negative control. Then you know what it is.

[Florian Scharhauser @FScharhauser]

@LPerepolkin I would increase the number of sequences if possible. Are there good quality sequences for the species you sequenced on NCBI already?.If not use some from the same genus. Try to have the same number of sequences in every group. Did you clean your alignment and trimmed it?

[Florian Scharhauser @FScharhauser]

@LPerepolkin @deejite1086 It could be that your primers are contaminated or your distilled water. I doubt it is the sensitivity of the primers. What is your PCR Master mix? Do you mix all the reagents ( dNTPs, MgCl2 ) individually or do you use a ready to use master mix?

[Florian Scharhauser @FScharhauser]

@deejite1086 @LPerepolkin I agree. What is your negative control and please check your PCR mix. You could run a PCR with blanks/ only the PCR mix without any DNA template and one positive control. When you get bands in all blanks it's very likely your reagents/ chemicals are contaminated.