Fotis Kyrilis

[Panagiotis Kastritis @3Dstructure]

Our new paper in #PNAS is out - see here: https://t.co/x2Ct71Ghjz! Congrats to @ToniTraeger for leading such a work, uncovering fantastic insights into active site regulation in isomerases from their endogenous state :) A small thread to explain how disorder regulates catalysis:

[Panagiotis Kastritis @3Dstructure]

We are excited to be opening 2 Ph.D. and 1 post-doc positions to work on cryo-EM and computational biology of plant protein complexes & their proteoforms (Projects A04, C04 and D01)!
Visit https://t.co/ocqy25c24N to be informed and apply ASAP! #SFB1664, headed by @quintlab

[Panagiotis Kastritis @3Dstructure]

And.... 11/11... here it is! @biorxivpreprint

https://t.co/ZoxkNDDJVu

[Panagiotis Kastritis @3Dstructure]

#KastritisLab strikes again: cell extracts and oxoglutarate dehydrogenase complex structure/function: Cryo-EM,cell biology,kinetics,XL-MS,Alphafold,HADDOCK: We visualise a gigantic 4 MDa active metabolon! paper is screened @BioRxiv; sneak-peak of main findings follow :-) (1/11)

[Ioannis Skalidis @imjohnskal]

MFW we start by cleaning the cryo-sample storage dewars but we end up dropping the coldest avant-garde (or classic) black metal album of the season #kastritislab #cryoem #labfun #phdlife #blackmetalaesthetic @K_Janson26 @hamdiphilum @Fotansky_16

[Ioannis Skalidis @imjohnskal]

At least I look decent in the photo!

[Panagiotis Kastritis @3Dstructure]

What about a #PhD in #KastritisLab, doing #cryoEM, #HADDOCK & #Alphafold to probe domain interactions regulated by disorder?
Apply until Feb 28 for the amazing #RTG2467 headed by @SinzAndrea, declaring your preference for Project 12! #phdlife https://t.co/FhUxj8So0K

[Panagiotis Kastritis @3Dstructure]

(1/10) We combined #AlphaFold and #CryoEM to derive atomic models of MegaDalton protein community members from cell extracts! The efforts from the #KastritisLab together with @ktototak were extraordinary to reveal their architectures - see thread below:
https://t.co/be3ypedACz

[Panagiotis Kastritis @3Dstructure]

(9/10) Our work shows the power of accurately modelling maps of intermediate resolution with #AlphaFold and #FindmySeq, revealing unique adaptations of native states - some required human intervention to resolve. We have a deep discussion on the impact of our work, please check!

[Panagiotis Kastritis @3Dstructure]

(10/10) I would like to thank @BMBF_Bund for funding our research, @dfg_public for supporting my lab, @UniHalle, as well as @embl for the amazing collaboration!! And of course, @imjohnskal @Fotansky_16 @ctueting1 @hamdiphilum @ktototak for producing such interesting story!!

[Ioannis Skalidis @imjohnskal]

It's finally out! Congrats to all for an amazing publication @Fotansky_16 @ctueting1 @hamdiphilum @ktototak @3Dstructure!
#kastritislab #cryoem #metabolons #alphafold #findmysequence

[Fotis Kyrilis @Fotansky_16]

Amazing cell biology research on moonlighting functions of OGDHc observing the inherent asymmetry of its subunits in muscles!

[n. @flymuscles]

Happy to share our new preprint! 🎉🥂
Oxoglutarate dehydrogenase coordinates myofibril growth by maintaining amino acid homeostasis https://t.co/LFWeiTXbUT

[Ioannis Skalidis @imjohnskal]

As @Fotansky_16 discovered today, even salt crystals seem to be in a festive mood with Christmas approaching! #christmasinthelab #kastritislab

[Panagiotis Kastritis @3Dstructure]

E3BP is not merely structural, and does not only bind the E3 - it is always in proximity of E2 monomers of the core, which are mostly in an open state configuration, showing functional implications of its positioning in the PDHc scaffold.
https://t.co/JumfTTxXAv

[Panagiotis Kastritis @3Dstructure]

We then saw that in the native PDHc core, not only the E3BP, that binds the E3 is inside the core, but it is occupied by protein densities up to ~20%, showing that the scaffold is filled with polypeptide regions and is not empty.
https://t.co/HCqdf1g9r5

[Panagiotis Kastritis @3Dstructure]

We characterized down to atomic resolution the acetylation of coenzyme A in the giant, native PDHc metabolon from eukaryotic extracts! Congrats to @ctueting1 @Fotansky_16 @hamdiphilum @imjohnskal @JMuellerBoerger @SorokinaMarija @YasharSadian , see thread

https://t.co/WKzZli13HB

[Ioannis Skalidis @imjohnskal]

New at Twitter @Fotansky_16 won the Best Poster Award at the #PDB50 #EMBLPDB conference, representing the #kastritislab and our amazing work! CONGRATS! Check out our publication that the poster was based on: https://t.co/pRXVrSY7Ll