The week ended realizing we had a wrong specification of barcode positions for DBiT @AtlasXomics
Fixed that, now everything seems working. Spatial GET-seq is now a few steps afar.
A detailed protocol for scGET-seq is now on @STARProtocols
We hope it’ll be useful for your research. Get in touch with @FrancescaGi82 (and myself) for any doubt
Analyzing genomic and epigenetic profiles in single cells by hybrid transposase (scGET-seq) https://t.co/PfG4Skn6Zz
How a genomic mutation change the transcriptome ?
How epigenetic changes affect transcripts levels and how those correlate with its translation?
Our review summarise the cool single cell multi-omic technologies available to answer those questions 😉
https://t.co/XO0BIEKCHR
If you're working on spatial transcriptomics, I think you'll find @LambdaMoses' "Museum of Spatial Transcriptomics", which analyzes the field via its metadata, to be an incredibly useful resource.
https://t.co/TYr8vyIVL6
It’s been a long journey, we had a lot of engaging scientific discussions and, more importantly, a lot of fun. I’ll end this 🧵with a picture dated 2019 which explains a lot of what was going on in the lab during scGET-seq development
To single cells then! @FrancescaGi82@lazarevi1965 and @MartinaTds hacked @10xGenomics scATAC protocol to multiplex the enzymes, tn5 and tnH are in the same tube (MEDs include a tn-specific barcode). We named this method scGET-seq (sc Genome and Epigenome by Transposases) 5/