Gallego Lab

Creativity and light microscopy to study cell growth
@UPFbiomed @the_prbb [email protected]

[Gallego Lab @GallegoLab]

1/10
In this thread we will explain you how we put together #SPT #SMLM #cryoET #cryoCLEM + #modeling to learn about the higher-order mechanism that controls constitutive exocytosis

(credits to Gallego lab/Dylan Godfrey)

[Gallego Lab @GallegoLab]

@SBEsp @CellCellPress @UPFBarcelona @qubilab @UVic_FCTE @uvic_ucc @BiofisikaScienc Thanks a lot @SBEsp for highlighting our paper and for promoting Biophysics in Spain!

[ann @annacb5_]

Join this amazing team at @UPFbiomed in Barcelona for a PhD decoding fungi evolution in extreme environments❄️ Cool microscopy with even cooler people!

[Gallego Lab @GallegoLab]

Please RT🙏

Our lab opens 2 PhD positions to investigate fungi's evolution:

Wet lab in Biochemistry/Structural Biology of cryo-tolerant fungi
https://t.co/lNd7ZceCen

Computational Physical Chemistry of cellular processes under extrem environments
https://t.co/WN8Pe5XYTD

[Gallego Lab @GallegoLab]

@MishaKudryashev @CellCellPress @_martapuig @sasmeek @JonasRies @carlomanzo78 @qubilab @dynamo_cryoET Thanks a lot Misha!:-)

[Gallego Lab @GallegoLab]

0/10
🥳 our #exocyst paper out in @CellCellPress, led by @_martapuig, Sebas & @sasmeek in collab with @JonasRies, @carlomanzo78, @qubilab
& @dynamo_cryoET

#Quantitative_Cell_Biology

Continuum architecture dynamics of vesicle tethering in exocytosis: https://t.co/z2HDrx0xFm

[Marta Puig @_martapuig]

Amazing example of team work! Big thanks to @sasmeek, Sebas, @GallegoLab and collaborators. Are you curious about the higher-order mechanism that controls exocytosis?👇🏼

[Gallego Lab @GallegoLab]

10/10
This was a true team effort, supported by our groups, institutions
@UPFbiomed
@UPFBarcelona
@uvic_ucc
@MaxPerutzLabs
@BasqueFor
funding agencies
@HFSP
@AgEInves
SNF,
collaborators, colleagues and reviewers that helped us to make it better

[Gallego Lab @GallegoLab]

9/10
The article leaves unanswered questions: How can exocyst tether vesicles at 45 nm of the membrane if its structure is 32 nm long? Why exocyst and SNAREs cohabit for 6 s? How Sec18 dismantles the ExHOS? Which is the role of metastable states and how is the transition ctrl?...

[Gallego Lab @GallegoLab]

Nuestro grupo oferta una de estas plazas! Si estás interesado o interesada tienes más información en nuestra web https://t.co/t0vVx8G0u9

[Agencia Estatal de Investigación @AgEInves]

🔬¡Oportunidad para jóvenes investigadores!

La convocatoria Proyectos Generación de Conocimiento (#PID) es la principal #convocatoria de investigación en España. En la PRP de 2024 se han concedido más de 3.000 ayudas y, entre ellas, se ofrecerán más de 1.000 contratos predoctorales para quienes quieran iniciar su carrera investigadora o realizar una #tesisdoctoral.

En este buscador puedes encontrar una lista con detalle de los #proyectos y donde está disponible el contrato predoctoral.
➡️https://t.co/pJEnhTrAeK

#FEDER #FSEPlus

[Biomedicina BCN @the_prbb]

📣 New PRBB-CRG conference

📋 Transmembrane domain-assisted folding of the integrin ectodomain
🗣️ Reinhard Faessler - @MPI_Biochem

  📆 May 26 - ⏰ 12:30 CET
hosted by: @GallegoLab - @UPFbiomed

📍#PRBB Auditorium

[Gallego Lab @GallegoLab]

Please RT🙌🤗
We just opened a PhD position to investigate biophysics in eukaryotes that grow in the cryosphere (glaciers, snow, Mars...)! If you like advanced imaging, evolution and quantitative biology, we are looking for you! Apply here👇
https://t.co/M7HFXMrn86

[Gallego Lab @GallegoLab]

This project was led by 3 amazing researchers: @_martapuig, Sebastian Ortiz and @sasmeek
with tons of help from our group at @UPFbiomed and the best collaborators ever:
@JonasRies, @carlomanzo78 and Daniel Castaño-Díez labs.
Thank you reader!

Feedback and RT appreciated

[Gallego Lab @GallegoLab]

Interested in imaging protein suprastructures and membranes dynamics in situ? Are you "preoccupied" by the exocyst? Read our latest preprint where we time-resolved the choreography of a flexible exocyst higher-order structure and the vesicle in exocytosis
https://t.co/WULdL6WTe6

[Gallego Lab @GallegoLab]

We were surprised that after fusion the ExHOS, with a radius of 38 nm, remains stable on the plasma membrane until it is disassembled by a reaction that requires the AAA ATPase Sec18! Which is the exact mechanism involved?? Well, this is hopefully to be shown in future threads