Hemanth Govinde

PhD Student

[Hemanth Govinde @HemanthGovinde]

@Dr_Biohacker Do look out for Simhasana (Lion Pose) or Khechari Mudra! One of the asanas in Yogasutras!

[Hemanth Govinde @HemanthGovinde]

@_peter_winslow_ No, we don't have a large proline seq in our protein.
But I really appreciate the suggestions, thanks

[Hemanth Govinde @HemanthGovinde]

Anyone with CD spec expertise?

I have 3 refolded recombinant proteins (40, 35 & 20 kDa) expressed in E. coli and purified using Ni-NTA. We performed CD analysis, but I’m stuck with spectrum interpretation/deconvolution and folding validation.
1/2

[Hemanth Govinde @HemanthGovinde]

@_peter_winslow_ Buffer - PBS
Protein conc. - 0.1 mg/ml
Path length - 2 mm
HTV is below 600 till 195, but goes beyond once we go below 195.

[Hemanth Govinde @HemanthGovinde]

@DNAutics I made this python based custom UI for plotting. If you see the raw CD signal, it's in terms of tens actually.
Pasted the formula that I used as well.
Btw thanks for replying! 🥹

[Hemanth Govinde @HemanthGovinde]

@DNAutics Thanks for the reply! Our proteins were dialysed against PBS. I have attached the CD spectra of one of the proteins. When I deconvoluted using free online tools, it says we have ~50% beta, ~10% alpha and rest random coils. Is it normal for a 40 kDa refolded protein?

[Hemanth Govinde @HemanthGovinde]

@ATinyGreenCell Hey just a question
How did you do the electroporation? Can you share the setup? @AmodRBhat built a DIY electroporator, but the transformation didn't work. (He has done it once but is not working right now) Thanks in advance 🥺

[Hemanth Govinde @HemanthGovinde]

@ATinyGreenCell Is the final version going to be a terminal app or are you planning to create a UI? Isn't a UI which opens on a separate window more user-friendly than the terminal app? 😬

[Hemanth Govinde @HemanthGovinde]

When the bands simulated on your own app actually match the real gel 👀
Feels incredibly satisfying.

https://t.co/N4e3ZkAy3J

[Hemanth Govinde @HemanthGovinde]

@yacineMTB Check my 2 recent posts😁

[Hemanth Govinde @HemanthGovinde]

@Mark1wan @ATinyGreenCell Thanks!!

[Hemanth Govinde @HemanthGovinde]

https://t.co/N4e3ZkAy3J

Vibe coded (Claude) an electrophoresis simulator.
Run the code and add more features if possible.

-Export PNG/SVG
-PCR mode - input both primers to simulate amplicon only
-Compare with multiple enzyme cuts (Use compare button) (1/n)

[Hemanth Govinde @HemanthGovinde]

@DNAwink thanks!!

[Hemanth Govinde @HemanthGovinde]

-Play between different gel percentages and buffers.
-You can add custom ladder
-Input voltage and run time to simulate your experimental conditions (2/n)

@AmodRBhat @ATinyGreenCell
Check this out

[Hemanth Govinde @HemanthGovinde]

@AndrewLipchik @KimbroughAlyssa @wsuapplebaum Hey! The CD spec data on the poster looks good. I am currently analysing CD spec data of my protein sample, and tbh I'm stuck. If you can help me, it would be really great. Please let me know how we can connect.

[Hemanth Govinde @HemanthGovinde]

@kakuwemu Yes. Please feel free to develop this code. We can get a better version with each update!!!

[Hemanth Govinde @HemanthGovinde]

https://t.co/eo4tiPnsvM

Vibe coded (Claude) PySide6-based molecular biology toolkit
Run https://t.co/ColKetvGL1 to launch the UI includes:
• Electrophoresis simulation
• Cloning (RE + Gibson Assembly)
• Primer design tools

@ATinyGreenCell
you might like this.

[Amod Raghupathi Bhat @AmodRBhat]

@district_india pathetic display of information in your app. Booked dhurandhar movie. Info showed hindi language and the app booked kannada tickets. Help me get my refund

[Hemanth Govinde @HemanthGovinde]

@ATinyGreenCell Waiting for the GitHub repo🥹🥹