ISIDORe

🇪🇺 ISIDORe has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101046133.

[LWNVIVAT @lwnvivat]

Some partners of our project took part at the @isidore_eu User Conference in Paris 🇫🇷 to discuss about pandemic preparedness and response👇

We participated in roundtable discussions to enhance global preparedness for future health emergencies.

[Patricia K. Carvajal @patricia_karvl]

Attending 2nd @isidore_eu user conference in Paris and glad to see @EatrisEric user’s community research results for Pandemic Preparedness

[Claudia Alen Amaro @claudialenamaro]

Peter Doran @ucddublin presenting the Joint Research Activities #JRA session @isidore_eu user meeting today in Paris

@instructhub shared their knowledge and expertise in three of these JRAs some of them will be presented today

We join Peter in thanking @ERINHA_RI coordination

[Claudia Alen Amaro @claudialenamaro]

Some hundred miles away we are discussing similar crucial issues in the context of the project @isidore_eu @ERINHA_RI director highlighting the importance of funding of research infrastructure for preparedness to health challenges both in Europe and the world
@instructhub

[Claudia Alen Amaro @claudialenamaro]

Great to read about @instructhub researchers! @isidore_eu Meeting give the opportunity to hear from users of other infrastructures
Instruct is always looking for synergies and services we can provide together
Just seen 2 posters of users of both Instruct-ERIC and @EuOpenscreen

[ISIDORe @isidore_eu]

🌍 At Institut Pasteur, we joined field training at MORU Bangkok with Prof. Phaik Yeong Cheah’s team to explore meaningful community engagement for #CCHF & #TBE. Practical insights to boost future preparedness & train tomorrow’s researchers! 👥🧪

#ISIDORe #PublicHealth

[ISIDORe @isidore_eu]

🧬 How do SARS-CoV-2 mutations boost fitness?

With #ISIDORe, Blackledge (IBS Grenoble) used high-field NMR to show that N protein mutations (e.g. Q229K, R203M) reshape structure & RNA binding.

Disorder = function.

#SARSCoV2 #Nucleocapsid #NMR #ViralFitness #ProteinDisorder

[ISIDORe @isidore_eu]

🦠 What does TBEV entry into neurons look like?

With #ISIDORe & CEITEC (Instruct CZ), Bisikalo (Helsinki) used cryo-ET to image TBEV on GLP2R-liposomes—confirming cryo-ET compatibility.

Next: higher-res data to reveal the virus–host interface.

#TBEV #CryoEM #Neurovirology

[ISIDORe @isidore_eu]

🫁 Can we prevent lung damage after TB?

Dr. Rath & OSPF (India) are launching a global Phase III trial testing metformin + NAC for post-TB recovery—thanks to #ISIDORe & #EATRIS support

✔️ TPP, trial design & WHO feedback secured.

https://t.co/Y9uDkTrSpV
https://t.co/QQxTQZjuOU

[ISIDORe @isidore_eu]

🧱 Can we break the TB barrier?

With #ISIDORe support, Kobakhidze (Max Perutz Labs) & EMBL Grenoble identified 2 fragments binding MmaA1, a key enzyme in Mtb’s lipid wall. One fragment shifts a critical loop—hinting at drug potential.

#Tuberculosis #DrugDiscovery

[ISIDORe @isidore_eu]

🧬 What do symptoms reveal about SARS-CoV-2 mutations?

In 428 healthcare workers, Milagro Beamonte’s team used Whole Genome Sequencing (WGS) to link S gene mutations (esp. in JN.1) with malaise.
WGS outperformed PCR for mutation detail—vital for real-time surveillance.

#ISIDORe

[ISIDORe @isidore_eu]

🧫 Can a DNA vaccine stop Klebsiella?

At Univ. of Girona, López Siles’ team is developing KlebsiGene to prevent drug-resistant infections.
With #ISIDORe/EATRIS, they confirmed no DNA integration & began transcriptome analysis.

#Klebsiella #DNAVaccine #AMR #VaccineDevelopment

[ISIDORe @isidore_eu]

🧬 How do viral & human proteins move?

With #ISIDORe, Spyroulias & Fourkiotis (U. Patras) used NMR to compare SARS-CoV-2 & PARP9 macro domains.

📍 Key finding: PARP9 MD2 shows dynamic flexibility—key to ligand binding & drug design.

#NMR #MacroDomains #SARSCoV2 #DrugDiscovery

[ISIDORe @isidore_eu]

🦠 New malaria drug target?
Kobakhidze (Max Perutz Labs) used ISIDORe & EMBL’s HTX Lab to solve the structure of PfSET7—revealing a parasite-specific pocket absent in humans.
🎯 A key step toward selective antimalarials.
#ISIDORe #Malaria #PfSET7 #DrugDiscovery #StructuralBiology

[ISIDORe @isidore_eu]

🦋 Silkworms for vaccines?

French biotech NEVEZYNE is developing a low-cost, scalable platform—starting with Ebola GP antigen.

With #ISIDORe & EATRIS support, they refined their regulatory strategy & TPP to prep for clinical steps.

#VaccineInnovation #Biomanufacturing #Ebola

[ISIDORe @isidore_eu]

🧬 Blocking poxvirus fusion = blocking infection.

With #ISIDORe, Guardado-Calvo’s team solved a fusion complex subunit bound to inhibitor & generated nanobodies targeting 4 subunits.
Next: test neutralization & solve top structures.

#Poxvirus #Antivirals #CryoEM #Nanobodies

[ISIDORe @isidore_eu]

🧪 What did we learn from #COVID-19 immunity in healthcare workers?

A study in Spain with #ISIDORe support found low memory T cell signals but broader CD4/CD8 shifts.
Despite limits, it informs post-vaccine immunity & feeds into long COVID & KAP studies.

#Tcells #PublicHealth

[ISIDORe @isidore_eu]

🧫 KlebsiGene: a DNA vaccine against Klebsiella.

López Siles (Univ. of Girona) advanced regulatory strategy & safety validation with ISIDORe, EATRIS & FIMM.
✔️ TPP defined
✔️ No DNA integration
✔️ Transcriptomics ongoing

#ISIDORe #DNAVaccine #AMR #Klebsiella #VaccineDevelopment

[ISIDORe @isidore_eu]

🧠 How do bunyaviruses evade host defenses?
Gerlach’s team (Polish Academy of Sciences), with #ISIDORe & EMBL, revealed how viral proteins mimic SG partners to hijack G3BP1—disrupting stress granules.

🔬 Crystal structures + cell imaging
📢 To be shared at EMBO/NSV 2025

[ISIDORe @isidore_eu]

🐖 What happens when pigs catch PRRSV vs. swine flu?
Bertho (INRAE/ONIRIS) found PRRSV triggers cytokines (IL-5, CXCL13, BAFF) but no neutralizing antibodies—unlike influenza.
➡️ Possible immune impairment during co-infection.
📢 BioRxiv Preprint
#ISIDORe #PRRSV #OneHealth