InCytometry - Graham Bottley

Providing training in Flow Cytometry for thousands of researchers since 2011.
Immunologist and Flow Cytometrist for 25 years.
ImmunologyExplained Patreon

[InCytometry - Graham Bottley @InCytometry]

A new ImmunologyExplained video looking at the common research and diagnostic technique of ELISA, used mainly to quantify antigen in a biological sample:
https://t.co/0AsGAMusta

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg That is irrelevant to your claim that it impacts immunity against other pathogens

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg I decided to correct your incorrect statement

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg But not for any others.

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg I pointed out a clarification to your post, so yes, I am helping. I have not said that there is no impact on immunity. ALL viruses have some form of immune evasion or suppression.

Do you think you are helping by misleading people?

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg And that is important when your posts are read by non-scientists.
If they interpret your posts as meaning that the SC2 virus can damage immunity against other pathogens, then you are misleading them.

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg I spend most of my time dealing with antivaxers, virus deniers, conspiracy theorists and cranks.
You are none of those.
However, scientific accuracy is absolutely vital, and there is a common claim that the virus causes general immune damage. It doesn't

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg I would agree that the virus can exhaust T cells against that infection specifically, yes.

[Rosewind @Rosewind2007]

I did wonder if using a picture of a bacteriophage would upset some virus deniers & (rolls eyes) yes it did…
A virus which infects bacteria is a virus—I also love their utterly funky appearance!
Here’s a picture of a difference virus, one which affects mammals, can you name it?

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg But potentially yes, very much so.

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg That depends on the time between infections, the severity of infection, the infectious dose the second time and whether there was viral persistence, which in itself is a major cause of T cell exhaustion

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg You said that it increased susceptibility to other infections and cancer.
So yes, you did.

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg See what you are doing is reading a paper without understanding the methodology or the immunological context, and then you are extrapolating that to a downstream effect that doesn't occur.

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg Oh my word.
Right.
There IS T cell exhaustion, but only in some clonal lineages. Not in all T cells.
That is a very, very important distinction.

And the papers you yourself link to show that. Have you not even read them?

[InCytometry - Graham Bottley @InCytometry]

@NickAnderegg No. You clearly don't understand the literature.

No literature anywhere shows general T cell exhaustion.

[Andrew Macdonald @OncoVirus_Leeds]

If you are interested in a Marie Sklodowska-Curie Fellowship application with our lab in tumour virology, please contact me. Example publications from the group https://t.co/L6CeYR0QXE and https://t.co/JPw4Nps4rH). #HPV #cancer

[InCytometry - Graham Bottley @InCytometry]

@FreeManOnEarth9 @smthngnope @m1sfitm9gn_t @Jhon50996854042 Explain then how HLA-restriction maps geographically to viral epitopes then.

[InCytometry - Graham Bottley @InCytometry]

At Cyto2024. I haven't been to a Cyto Conference for a few years, so it is nice to catch up with lots of other cytometrists!

[InCytometry - Graham Bottley @InCytometry]

@smthngnope @Jhon50996854042 @FreeManOnEarth9 Amazing, isn't it.
I have made over half a million £ from that hobby so far, doing something I really enjoy, and with a worldwide appreciative audience.
They somehow see it as a negative thing.

Says a huge amount about their own outlook on life.