JStuttmann

Plant molecular biologist interested in biotechnology and plant-microbe interactions.

[JStuttmann @JStuttmann]

En 11/2023, j'ai fait la demande pour l'immatriculation d'un vehicule chez @FranceTitres. Toujours rien. Hereusement, le délai de traitement (pyrenees orientales, 66) n’est pas encore dépassé.
J'adoré "la France sans papier", felicitations. Interessant pour @MadeInPerpi?

[JStuttmann @JStuttmann]

https://t.co/Iwm29UZJKg

[JStuttmann @JStuttmann]

@ANTSdirect
Ma demande d'immatriculation va bientôt fêter son premier anniversaire. Info sur un délai pas possible. En instruction, tout va bien. Faut-il se contenter avec ça?

[Tom Schreiber @cbtomsen]

I am happy to share our engineered CRISPR-endonucleases favoring homology-directed repair in plants. This tool is particularly useful for precisely inserting gene-sized DNA payloads. Big thanks to all involved! 🧬👏🏼 https://t.co/NqY2r6L4lC

[El Kasmi Lab @Farid_El_Kasmi]

I am very proud to see this super interesting work of my PhD student Sruthi online now!!👍🏽😃
Congrats and thanks to all involved!
https://t.co/Bcz76br3me

[JStuttmann @JStuttmann]

Registration link:
https://t.co/IlTSkmnNgW

[Tom Schreiber @cbtomsen]

Interested in the design and application of #CRISPR -based tools in plants? Join the masterclass.

[JStuttmann @JStuttmann]

Hello everyone, help! We have an older Olympus fluoview 10i system, but the corresponding interface card (PCIe) is broken. Maybe someone could have a card like this from a system that is not used anymore? From Olympus, it's 2k. Please retweet!

[JStuttmann @JStuttmann]

@YvonJaillais Hi, there was a promoter from arabidopsis in a presentation one day that was apparently expressed in the entire root, but not shoot. ARSK1 if I remember right. We wanted to clone it one day, I can find details, if you like.

[JStuttmann @JStuttmann]

Our small contribution on genome editing with @JanaOrdon now published: https://t.co/JyWtuf7Pg1

2 🔑messages
1) TREX2 can make your editing ~ 2 x more efficient
2) More guide RNAs can make your life easier when you want to induce deletions of chromosmal segments.
Thanks to all!

[JStuttmann @JStuttmann]

https://t.co/iWPlJIi7x4
I am puzzled by results on EDS-SAG subcellular localization requirements. We did similar experiments [doi:10.1111/nph.18511] - NES may be confounding, but we did not see reduced function of E/S-NLS versions. Not the same species, but still very surprising.

[JStuttmann @JStuttmann]

it does not change deletion frequency, but point mutations increased by two-fold. Not new, TREX2 was used in several papers with @DanVoytas. But we put a number on it in https://t.co/r3ZrzBoKDA. by a direct comparison in stable transgenics. I hope this can be helpful for some.

[JStuttmann @JStuttmann]

A small CRISPR-technical manuscript with @JanaOrdon:
https://t.co/PDOJmhDHIt
We tested induction of chromosomal deletions. Apparently, frequency is higher when multiple sites flanking the fragment are targeted. Further, we tested TREX2 co-expression (with Cas9) in Arabidopsis...

[JStuttmann @JStuttmann]

@mikrobenjaeger @AmreiBahr Das Problem ist bloß, dass das Einwerben von Drittmitteln (welches auch ich, wenigstens in unserer Disziplin, als Schlüssel sehe), ebenfalls den Faktoren Kontakte, Glück und Timing unterliegt. E13 Dauer ist schön und gut... und wird meist basierend auf Sympathien besetzt.

[JStuttmann @JStuttmann]

@IzzySaur Hi, the Xanthos I worked with were very happy with NYG medium, solid or liquid. As an alternative to competent cells, triparental conjugation works fine. In most cases, there is no point in going liquid, they are more happy on plates.

[JStuttmann @JStuttmann]

@MPPjournal I do not have a substantiated opinion as not my topic. But would would just like to refer also to this preprint for possible debate, as I think it is not cited in the MPP article https://t.co/yauQxrBYQK

[JStuttmann @JStuttmann]

So many thanks to reviewers, and to NPH and editor for handling this; paper changed substantially between first submission and revisions. And many thanks also to collaborators and contributors. I think there is interesting info for some in the field.

[JStuttmann @JStuttmann]

I am very happy to share the last results from my little team in Halle (does not exist anymore) in NPH.
https://t.co/LjBLm3CvqC
A bit of a potpourri... we looked at PRR and TNL functions in Nbenth. Different from At, we do not detect a role of EDS1 complexes in Nb PRR signaling.

[JStuttmann @JStuttmann]

Always much criticism on peer reviewing, and I may also have been dissapointed before... but it really did a great job on this one. The reviewers proposed important experiments that cleared out last doubts on significance of the EDS1 triad in TNL-ETI.👍👏