Jalink Lab

Functional imaging lab where we combine cell biology with live cell imaging and biophysical tools. #FRET #FLIM
PI - @jalink_kees @NKI_nl
Tweets by lab members

[Jalink Lab @JalinkLab]

Super happy that we have finally put this work out!!! 4 years in the making:
We present – DynaScreen: an imaging (FRET-FLIM) based pooled CRISPR screening platform for studying “dynamic” phenotypes. short🧵below (1/n)

[bioRxiv Cell Biology @biorxiv_cellbio]

Beyond Static Screens: A High-Throughput Pooled Imaging CRISPR Platform for Dynamic Phenotype Discovery https://t.co/jEpbmUELXE #biorxiv_cellbio

[Jalink Lab @JalinkLab]

Latest paper from our lab! 🔬

[FEBS Open Bio @FEBSOpenBio]

Better tools = better science = better medicines. 🧬✨

💡THE IDEA: create a huge mixed batch of cells where each cell has a different gene switched off. Then scan through them under a microscope, looking for anything unusual and when you find it, mark it with a laser so you can pick it out later.

👎THE PROBLEM: the laser light was too intense meaning the tag was switching on and then immediately burned out.

👍THE FIX: turn down the laser power, reduce how long it lingers on each spot, and slightly blur the focus.

Simple tweaks but the result is cells that glow much more brightly and reliably helping scientists accurately track cells of interest.

Interested in learning more? Have a look at the Research Protocol from @sravmukherjee , Giulia Zanetti, Bram van den Broek, @jalink_kees from @NKI_nl and @UvA_Amsterdam

🔗https://t.co/HBNO9cMbGH

[Jalink Lab @JalinkLab]

Its a short and sweet paper summing up the hurdles, how to overcome them and tips and tricks to get best photoactivation results led by @sravmukherjee.

Happy reading!

[Jalink Lab @JalinkLab]

📢 Hot off the @FEBSOpenBio press, our labs' newest paper - "Optimizing photoactivation of PA-mCherry for optical pooled CRISPR screens"
https://t.co/2OWX5aPWl5 🍒⬇️⬇️

[Jalink Lab @JalinkLab]

- Designed to mimic widefield activation in as much as it spreads out the excitation energy over the cell, both in space and in time, we dubbed this method ‘pseudo-widefield’ scanning.

[Jalink Lab @JalinkLab]

If you have seen @sravmukherjee or Kees presenting this work at multiple conferences – this is the final product of our beloved project. We are looking forward to perform many more screens with this robust pipeline. Please give it a read & we are very happy to receive feedback!

[Jalink Lab @JalinkLab]

Super happy that we have finally put this work out!!! 4 years in the making:
We present – DynaScreen: an imaging (FRET-FLIM) based pooled CRISPR screening platform for studying “dynamic” phenotypes. short🧵below (1/n)

[bioRxiv Cell Biology @biorxiv_cellbio]

Beyond Static Screens: A High-Throughput Pooled Imaging CRISPR Platform for Dynamic Phenotype Discovery https://t.co/jEpbmUELXE #biorxiv_cellbio

[Jalink Lab @JalinkLab]

💡We successfully optimised samples for NGS with extremely low cell counts (~200 cells)
💡We show why single cell measurements (via imaging) are important in comparison to bulk cell measurements (ELISAs/Western Blots) (8/n)

[Pablo Munoz @pablo_jack]

Brilliant work improving the Epac based biosensors. During my pHD in Groningen I used Jalink biosensors and they always deliver great result and Jalink lab always help with any doubts and share them.

[Jalink Lab @JalinkLab]

🥳🥳🥳 let's go team!! 🤓🔬🧫

[Sravasti Mukherjee @sravmukherjee]

Back in June this year, I had the opportunity of giving a talk at the 17th @ipscconf organized by the MDC in Berlin!!!
I also won the best talk award which was the cherry on top!!! 🤓

Thanks to the organizers for a great conference!!! Look forward to next year!! 🥳

[Jalink Lab @JalinkLab]

🔬 We are excited to make these powerful tools available to the microscopy community and look forward to seeing how they enable deeper insights into cellular signaling and real-time dynamics in diverse research applications!!!

[Jalink Lab @JalinkLab]

Our latest preprint from the lab is now OUT!!! 🧫🔬

Spearheaded by our PhD student Giulia Zanetti - we made cytosolic-enhanced dedicated FLIM sensors for intracellular cAMP detection!

Check it out and a small thread below 👇

[bioRxiv Cell Biology @biorxiv_cellbio]

Cytosolic Enhanced Dark Epac-Based FRET Sensors for Intracellular cAMP detection in live cells via FLIM https://t.co/ZxSwrGY5ar #biorxiv_cellbio

[Jalink Lab @JalinkLab]

These sensors, Epac-based, show improved cytosolic localization while maintaining excellent performance. They are ideal for co-expression with other FRET/FLIM sensors, offering greater flexibility in live-cell imaging experiments.