Jing Karchin

Mom 👩‍👧| PhD scientist |Project manager in Tasian team | CRC @PKIDS_Network @CHOP_CPCE | Educator | Cat enthusiast | she/her | Views my own

[Alice Ting @aliceyting]

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec.

We explore 4 intracellular and intercellular applications of TransitID:
- discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix)
- mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress
- discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery
- analysis of proteins that traffic intercellularly bw tumor cells & macrophages

Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor

https://t.co/yo4laOA14z

[Jing Karchin @JingBibi]

@Yueyao_Zhu Good luck Yueyao!

[Jing Karchin @JingBibi]

@UpstateCDBio Well deserved!! Congratulations Mira!! @KidneyMyosin Very happy for you!

[Jing Karchin @JingBibi]

@sanasuri @Momademia Bring a car seat on the plane. It’s a hustle to bring but it’s way easier for him to sit still and be contained. Please it’s safer to sleep in it.

[Patrick F. Bloniasz (he/him) @PatrickBloniasz]

[1/n] I am offering a free Zoom "Intro to R" data wrangling workshop on January 14th, 2023 after popular demand. Specifically, I will go through the use of the Tidyverse. All disciplines and beginner* R skill levels are welcome. @PhDVoice @AcademicChatter @OpenAcademics

[Jing Karchin @JingBibi]

@Yueyao_Zhu Congratulations! Hard work paid off!!

[Jing Karchin @JingBibi]

@mayanorthen Happy birthday!!

[Jing Karchin @JingBibi]

@KidneyMyosin Love the names!!

[Jing Karchin @JingBibi]

@KidneyMyosin @PeiJuLiu1 @PSUresearch @BostonChildrens @Claire_EMartin_ @MichaelGarone Congratulations again! Great collaboration indeed!

[Mira Krendel @KidneyMyosin]

Our new study of MYO1E variants associated with nephrotic syndrome! @PeiJuLiu1 led a great collaboration with several labs, incl. Chris Yengo's lab @PSUresearch, Friedhelm Hildebrandt's lab @BostonChildrens, @Claire_EMartin_, & several Krendel lab members, incl. @MichaelGarone.

[Jing Karchin @JingBibi]

@adrimantegazza @TJU_Micro @KHamiltonPhD @zdhutchins @EScharrig Congratulations to the team!!

[Jing Karchin @JingBibi]

@mayanorthen @baugelli78 Happy anniversary!! I hope you guys have a fun weekend to celebrate!

[Jing Karchin @JingBibi]

@jenniejlin I’m so sorry Jennie!! How are you and Calvin doing?

[Jing Karchin @JingBibi]

@marcotrizzino Congratulations! This is very exciting! Best of luck!

[Jing Karchin @JingBibi]

@pragati2707 @MITKavli @ERAUPrescott @astro_noel Congratulations! Good luck with the move and everything!!

[David Morris @wdmorrisjr]

As a parent, you have to pick your battles.

These are some stands I've been glad we've taken.

They're not easy, but they're worth the fight.

As I often say to my kids ⏤

"I know you don't think this is important,

But one day you'll be so thankful you had parents who did."

[Jing Karchin @JingBibi]

Seriously I don’t want to jinx this but I can’t wait for this to pass the House.

[Eric Feigl-Ding @DrEricDing]

BREAKING—a bill to make Daylight Savings permanent just passed the US Senate by **Unanimous Consent** (S.623: Sunshine Protection Act, as amended to make Daylight Saving Time permanent). It only needs to now pass the House before going to Biden’s desk!

[Jing Karchin @JingBibi]

@Yueyao_Zhu Do it!! 😻😻

[Jing Karchin @JingBibi]

Sunset on #onehappyisland

[Penn Urology @PennUrology]

Now accepting apps for our funded 2-month summer internship for 1 MD/DO or MS/PhD student to work with us @GregoryTasian @Justin_Ziemba @Penn @ChildrensPhila in applying machine learning tech to CT image analysis in renal stone disease. For more info/app https://t.co/fXE3UbDtCH