@MesenchymalCell Primary MSCs do not seem to proliferate to any great extent when cultured continuously in a hollow fibre bioreactor. Large amounts of EVs can be regularly collected. https://t.co/7egoapeBAQ
@C_Balbi90@MartaAdamiak Purity of EVs from a hollow fibre bioreactor shown to be superior to that obtained with flasks. Possibly helpful? https://t.co/63QBFfjsbC
@flscientist@KennethWWitwer @IsevComms @ISEV_JEV @BLehrich @cnssurgeon fyi - for cell lines maintained at high density >=10^8 cells/ml you can generally eliminate serum using CDM-HD.
@BGiebel@KennethWWitwer We have had reports that EVs from hollow fibre supernatants can be 10x higher in purity than from flask, so offering a good starting point, potentially.
“If you thought Covid was bad, you don’t want anti-microbial resistance. I don’t think I’m exaggerating to say it’s the biggest human health threat, bar none. Covid is not anywhere near the potential impact of AMR.” https://t.co/qeNv867BQN #StopSuperbugs
@DrTomWhittaker @BGiebel perhaps helpful: you can choose between 20 kDa and 5 kDa mwco fibres in a Fibercell hollow fibre bioreactor. The 5 kDa version has been used to prepare secretome for proteomics analysis and biomarker discovery. 20 kDa version good for high yields of EVs. https://t.co/u68R3kEKfW
Congratulations to our Project Coordinator @lodrisc1 on the publication of "Extracellular Vesicles from Mesenchymal Stem Cells as a Covid-19 treatment". Access the article using the following link:
https://t.co/K4HJkDTLRj
@TCDPharmacy@tcdTBSI@tcddublin
TRAIN-EV ESR @SaraiMrtnez, with the supervision of our Project Coordinator @lodrisc1, has designed a survey on the use of in vitro preclinical models used in cancer research and would appreciate your input. Please RT
https://t.co/J2FK84lnA2