Akhilesh Pandey Lab

Improving and individualizing medicine through translational systems biology. Mass spectrometry, Multi-Omics, Diagnostics. Mayo Clinic. Views are personal.

[Akhilesh Pandey Lab @Lab_Pandey]

Significant decrease in N-glycopeptides was seen in haptoglobin, ceruloplasmin and albumin among others. These site-specific glycopeptide changes may provide new biomarkers and insights into SRD5A3-CDG pathophysiology. Led by Anu Jain @Dr_Anu_Jain from our team.

[Akhilesh Pandey Lab @Lab_Pandey]

Just out in Journal of Inherited Metabolic Disease @JIMD_Editors: our study on multiplexed mass spectrometry-based glycoproteomics of serum proteins in SRD5A3-CDG, a disorder of dolichol synthesis. We found hypoglycosylation across the serum glycoproteome. https://t.co/j1d0XMEAy8

[Akhilesh Pandey Lab @Lab_Pandey]

This method enables detection of multiple clinically relevant markers at once in a single #FFPE section. It is concordant with IHC and offers new possibilities for multiplexed spatial biomarker resolution. Led by Kiran K. Mangalaparthi @mkirankumar89 and Daigo Gunji from our team

[Akhilesh Pandey Lab @Lab_Pandey]

Excited to share our latest work demonstrating an imaging mass spectrometry workflow combining #MALDI with #IHC. We deployed photocleavable mass tag-conjugated antibodies to analyze spatial distribution of ER, PR, and HER2 proteins in breast cancer https://t.co/KRzIiGycdF

[Akhilesh Pandey Lab @Lab_Pandey]

Congratulations to Kishore Garapati @kilroykishore Anu Jain @Dr_Anu_Jain Neha Joshi @Salvation_007 and others from our team and to our collaborators!

[Akhilesh Pandey Lab @Lab_Pandey]

Excited about our research now published in Molecular Genetics & Metabolism! N-linked glycosylation of albumin is altered in congenital disorders of glycosylation with #biomarker potential. #CDG https://t.co/jEz3P6JKus

[Akhilesh Pandey Lab @Lab_Pandey]

Albumin carries N-glycans at two non-canonical sites: N68, N123. We show changes in albumin glycopeptides consistent with biochemical defects in type I, II and mixed type CDG. These new targets may complement transferrin testing in diagnosis and monitoring. #RareDisease #MassSpec

[Akhilesh Pandey Lab @Lab_Pandey]

These extra findings (IGH rearrangements, TP53 loss, 1q gain, chromothripsis) can alter risk stratification and treatment decisions. Proximity mapping may improve diagnosis and enable more precise, mutation-guided therapy. Led by Richard Kandasamy @richardKumaranK from our team

[Akhilesh Pandey Lab @Lab_Pandey]

Published in the American Journal of Hematology @AjHematology: our work on multiple #myeloma showing Genomic Proximity Mapping can confirm FISH findings and also detect clinically relevant structural variants, CNVs, ploidy changes https://t.co/yKiBza7z5e

[Akhilesh Pandey Lab @Lab_Pandey]

Using targeted mass spectrometry-based measurement of all hemoglobin chains, we discovered that the levels of #hemoglobin mu chain are increased in patients with alpha thalassemia where the HBM gene is spared from deletion, indicating a compensatory role and #biomarker potential

[Akhilesh Pandey Lab @Lab_Pandey]

Excited to share our research on the mu chain of hemoglobin published in Blood Red Cells & Iron @BloodPortfolio! Once considered a pseudogene, we show that HBM encodes a protein that is produced in gestation at levels similar to embryonic globins https://t.co/axWpIRHy8a

[Akhilesh Pandey Lab @Lab_Pandey]

We present a targeted strategy combining tandem mass tag multiplexing with SureQuant-based triggered MS/MS using super heavy TMT-labeled peptides as triggers, enabling robust quantitation of histone PTMs with improved analytical throughput #Singlecellproteomics

[Akhilesh Pandey Lab @Lab_Pandey]

Happy to share our study on a novel approach for targeted measurements of post-translational modifications of single cells published in Journal of Proteome Research @JProteomeRes and led by @DongGimun from our team https://t.co/PeKqe92Pii

[Akhilesh Pandey Lab @Lab_Pandey]

Immune proteins rise with severity while metabolic proteins rise early, and are potential #biomarkers for early detection of plasma cell disorders. Led by Joel-Sean Hsu @hsu_joelsean and Richard Kandasamy @richardKumaranK from our group https://t.co/qSbjDz7LnI

[Akhilesh Pandey Lab @Lab_Pandey]

Excited to share our new study published in Blood Cancer Journal @BloodCancerJnl! Using @OlinkProteomics we profiled bone marrow plasma from #MultipleMyeloma & monoclonal gammopathy of undetermined significance patients to uncover specific protein signatures #MyelomaResearch

[Mayo Clinic @MayoClinic]

.@MayoCancerCare researchers will present their latest radiation oncology findings at the 2025 American Society for Radiation Oncology (ASTRO) Annual Meeting, scheduled to be held Sept. 27–Oct. 1 at the Moscone Center in San Francisco.

Learn more: https://t.co/RTI4nCbhWD

[Akhilesh Pandey Lab @Lab_Pandey]

Congratulations to Kishore for presenting our glycoproteomics work in congenital disorders of glycosylation at the Ehlers Symposium & BMB Research Retreat at Mayo Clinic — and for winning a Best Poster Award! 🏆 Exciting recognition for outstanding science! @kilroykishore

[Akhilesh Pandey Lab @Lab_Pandey]

Shoutout to Joel-Sean Hsu from our team for presenting our ongoing research on IMiDs in multiple myeloma at the Ehlers Symposium & BMB Research Retreat at Mayo Clinic. Grateful for the opportunity to share our work and connect with the community 🧑‍🔬🧪

[Akhilesh Pandey Lab @Lab_Pandey]

Check out Dong-Gi Mun’s poster in applying super heavy TMT to single cells 🧫

[Akhilesh Pandey Lab @Lab_Pandey]

Proud to present our studies on human brain peptidomics and terminomics by Hiroshi Nishida 🧠 #ASMS2025