Stephanie Linehan

Molecular Analyst 🧑‍🔬 | Scuba Diver 🌊 | Bunny Lover🐰 | Foodie 🍴|

[Rathlin Stickybeak @_Stickybeak]

Hare one moment, gone the next 😉 Irish Hares are showing themselves more frequently these days but the typical encounter doesn't last long

[This is Galway @ThisIsGalway]

Brilliant stuff from @ConnachtHotel as they offer their beautiful space The AVIARY for local remote workers who are without Wi-Fi or electricity at the moment... you can even avail of complimentary Tea & Coffee while you work! ✨🙌👏

More here: https://t.co/x1NRPsQu97

[Stephanie Linehan @LinehanOx]

@DublinAirport @aegeanairlines #GreekGetaway 🙏

[Stephanie Linehan @LinehanOx]

I'm sure there is still a car along the prom somewhere! 😅

[Galway Atlantaquaria @GalwayAquarium]

Matt & all the animals are still ok. I did panic a bit when I couldn’t reach him for 30 mins but it turns out he had managed to fall asleep for a few mins & then I woke him up in a panic! 😂
You know it’s bad when a large wooden bench is seen ‘walking’ down the prom! #StormÉowyn

[Met Éireann @MetEireann]

Red warnings for #StormÉowyn have been updated as follows.

These changes are because the latest forecast shows the system clearing out slightly earlier than forecasted yesterday.

[Father Ted Quote of the Day @FrTedQOTD]

"God Ted, it's no use. I'm just so excited about Christmas I can't sleep."

[Stephanie Linehan @LinehanOx]

A great intro to fish pathogen genomics hosted by @EAFP_news & @CefasGovUK in the @roslininstitute funded by SAIC. Thanks to everyone who organised & spoke at the workshop. It was so valuable to get hands on experience with coding and using various bioinformatic applications! 🦠

[Chantelle Hooper @ChantelleHooper]

We had a great turnout for the @EAFP_news fish pathogen genomics workshop earlier this week! The participants went from command line basics to identifying and characterising a bacterial species causing disease in fish from a whole genome sequencing dataset.

[KNEECAP @KNEECAPCEOL]

Today we took some of the King’s treasure and we gave it to kids in Belfast, on both sides of the community!

[Bento Lab 🧬 @theBentoLab]

Did you know that microwaving to burst open cells can be a useful method of DNA extraction, especially for cheap, rapid, and high-throughput workflows?

It can be used as a step within the extraction process to improve cell lysis, and also as the main cell lysis process to produce a crude DNA extract for PCR.

An early example was published by Goodwin & Lee (1993), and was used on a wide range of taxa across different kingdoms of life:

⭐Goodwin & Lee (1993). Microwave miniprep of total genomic DNA from fungi, plants, protists and animals for PCR. Biotechniques, 15(3), 438-441.
https://t.co/TsI49uyNd1

Since then, researchers have regularly published variations or instances of this method for specific applications. Below are a few examples 👇

⭐DNA extraction from human samples (saliva, blood, semen) for forensics:

Taglia et al. (2022). Development of a microwave-based extraction for forensic biological samples. Forensic Science International: Synergy, 5, 100291.
https://t.co/i69tJlk1x2

⭐High throughput DNA extraction from barley seeds for genotyping, using microwaving in an alkaline buffer followed by neutralisation with a Tris-HCl buffer, allowing processing of thousands of seeds per day:

Von Post et al. (2003). A high-throughput DNA extraction method for barley seed. Euphytica, 130, 255-260.
https://t.co/MloxBWwM9E

⭐Microwaving dry fungal spores to pop them open prior to direct PCR:

Ferreira et al. (1996). PCR from fungal spores after microwave treatment. Fungal Genetics Reports, 43(1), 25-26.
https://t.co/8W1adScQ9y

⭐Microwaving ground mosquitos, in which it was found to produce comparable results to much more time-consuming extraction methods:

Firmansyah et al. (2023). Comparison of 3 DNA extraction methods for extracting DNA from an adult Culex quinquefasciatus (Diptera: Culicidae). Journal of Insect Science, 23(5), 15.
https://t.co/lL1H6rmL65

Image shows a microwave oven, CC BY-SA Mk2010, https://t.co/E6bOV45B4F

Do you use microwaving as part of your DNA extractions? If you do, we'd love to hear about how it works for you!

[𝗚𝗮𝗹𝘄𝗮𝘆 𝗧𝗼𝘂𝗿𝗶𝘀𝗺 @galwaytourism]

Pumpkin supermoon over Inisheer Lighthouse, Aran Islands, Galway 🎃🌕🧡

#Supermoon #LoveGalway
📸 ig/felix.sproll

[Stephanie Linehan @LinehanOx]

Itching to get back to the @MarineInst molecular labs after a fascinating and informative course on digital PCR #dPCR at @ugent It was a great opportunity to meet other people from different sectors starting to use dPCR in their work and their experiences with it! #digPCR #Ghent

[Elvira de Eyto @edeeyto]

Great excitement here to today when @KeenaPhilip spotted some of our Freshwater Pearl Mussels releasing glochidia into the fish tanks. The salmon 🐟was having a taste. The 2nd 🎥 is of snapping glochidia under the 🔬 looking for a fish.
#CaptiveBreeding
@MarineInst @NPWSIreland

[Stephanie Linehan @LinehanOx]

So happy to see you guys recognised for all your hard work and the acknowledgement that your chocolates are the best 😋😋 #bonchocolatiers

[Bon Chocolatiers @bonchocolatiers]

To top it all off, our Truly Dark has also been nominated for the Golden Fork, which is known as “the Oscars of food & drink”. There are only 3 nominees from Ireland this year and we are so excited to represent Ireland on the world stage at the awards ceremony in London 🌟🤎

[Philip Keena @KeenaPhilip]

Want to get training using underwater drones or need services carried out using this equipment get in touch at https://t.co/E2A5jVmSzc to see how we can help 👍 #exploringunderthesurface

[Stephanie Linehan @LinehanOx]

Nice discussions at the Fish Handling Biosecurity Training in Newport yesterday @MarineInst @Zoetis

[Marine Institute @MarineInst]

🐟Fish Handling Biosecurity training at the Marine institute in Newport given by our designated Veterinarian Dr. Susie Mitchell of Zoetis Ireland Ltd.

Staff and bursars of the Newport Rearing facility, the Fish Health Unit, and our fish tagging team attended the training.

[Stephanie Linehan @LinehanOx]

Such an awful situation but great to see the community come together

https://t.co/jAihjp6iJi

[Marine Institute @MarineInst]

🐟Busy day in the Furnace, Newport hatchery for our @MarineInst captive breeding program for freshwater #PearlMussels. The team are looking for #glochidiosis (larval mussels attached to the gills) in the 2023/2024 cohorts of salmon and trout. All fish are measured and checked for health status, with thanks to our colleagues in the Fish Health Unit.

@NPWSIreland @NPWSNatureCons

[Stephanie Linehan @LinehanOx]

Some brilliant talks from colleagues in the @MarineInst for @pintofscienceIE event in #Galway Highlights for me were ships, sharks and sneaky waves #pint24

[Stephanie Linehan @LinehanOx]

Still can't believe we saw the #Aurora this weekend in #Donegal 🌌

[RTÉ News @rtenews]

Electronic shock collar for dogs and cats that are controlled using hand-held remote controls are to be banned in Ireland following a public consultation ordered by the Minister for Agriculture
https://t.co/YDjidiqFbM