Maurice Filo

Scientist (ETH) | Masters & PhD, Mechanical Engineering (UCSB) | Masters, Electrical Engineering (AUB) | Control Theorist | #SyntheticBiology | Dad & Pianist |

[Maurice Filo @MauriceFilo]

Just published: "Inteins: A Swiss Army Knife for #SyntheticBiology" with @St_Anastassov & @KhammashLab in Biotechnology Advances.
One day, #Inteins 𝐜𝐨𝐮𝐥𝐝 make #genetic circuit design as simple as electric ones! 🧬⚡ https://t.co/en8cziEXTi

[Maurice Filo @MauriceFilo]

We have crafted 3 different topologies that implement various anti-windup strategies. We developed a couple of theorems that link this molecular strategies to well-known control-theoretic strategies. We also devised reaction network and genetic realizations using inteins.

[Maurice Filo @MauriceFilo]

Check out our paper in the @KhammashLab
on #biomolecular protection circuits. Integral windup is similar to a trauma inflicted by severe disturbances with lasting effects even after the disturbance ends. How do we mitigate that in biomolecular circuits?1/n https://t.co/7F3S8ov380

[Maurice Filo @MauriceFilo]

An anti-windup strategy is like a therapeutic intervention, helping a controller to move past its traumatic experience by promoting a form of forgetfulness toward the disruptive event. Anti-windup circuits thus avoid windup-induced instabilities and poor dynamic performance. 2/n

[Maurice Filo @MauriceFilo]

@AwadMariette Congrats!

[Maurice Filo @MauriceFilo]

@mariotelfig This gives a whole new meaning to "thinking out of the box"!

[Mario Figueiredo @mariotelfig]

My favorite illustration of how geometric intuition fails miserably in high dimensions.

[Maurice Filo @MauriceFilo]

@JuncalArbelaiz @Ashesi @ETH4D It was indeed!

[Maurice Filo @MauriceFilo]

One of the highlights of 2023 is teaching #controlsystems to an incredible cohort at @Ashesi University in #ghana 🇬🇭 as part of the @ETH4D (ETH for Development) master program. It was an absolute pleasure getting to know all of the students!

[Mustafa Khammash @KhammashLab]

Are you looking for a versatile and inexpensive illumination platform for optogenetics experiments?

Check out our new DIYA device... It's become the most popular platform in our lab for bacterial, yeast and mammalian optogenetics.

#synbio #optogenetics

https://t.co/txk715XLFH

[Maurice Filo @MauriceFilo]

@FrancoLabUCLA Too many legends in one panel! (This is reviewer 2 speaking) 😂

[Maurice Filo @MauriceFilo]

Reference-based antithetic integral feedback (AIF) was previously shown to deliver robust perfect adaptation at the population level, but may increase cell-to-cell variability. The sensor-based AIF, however, is capable of reducing variability, thanks to precisely the hidden P!

[Maurice Filo @MauriceFilo]

Check out our latest preprint in the @KhammashLab on "A Hidden Proportional Feedback Mechanism Underlies Enhanced Dynamic Performance and Noise Rejection in Sensor-Based Antithetic Integral Control"
https://t.co/LdzOQZg8Pi
Special thanks to my coauthor @MucunHou

[Maurice Filo @MauriceFilo]

Actuating by the sensor molecule Z2 (instead of Z1) embeds a (filtered) PI controller in just one actuation reaction. This brings with it the enhanced dynamic performance offered by the P component while maintaining the robustness offered by the integrator.

[Maurice Filo @MauriceFilo]

@AptaWineClub That’s what I miss most about California!

[Maurice Filo @MauriceFilo]

@NaderLikeLadder 😂very acurate. You know that it’s useless and a sort of a waste of time, but still you do it because “3ayb”.

[Maurice Filo @MauriceFilo]

Fast post-translational modifications performed by inteins may generate sophisticated #controllers. To study and untangle the underlying dynamics, we have developed two theorems that enable a graphical screening for #RPA and a #modelreduction recipe accessible to non-experts 5/5

[Maurice Filo @MauriceFilo]

Check out our work in the @KhammashLab on building #integral controllers genetically with split #inteins! (Play the video with sound on for an animation of a genetic circuit!)
https://t.co/peQ2H3TrmH
Special thanks to my coauthors @St_Anastassov, @Ch_Hs_Chang & @KhammashLab 1/5

[Maurice Filo @MauriceFilo]

Multiple #genetic #circuits, with remarkable dynamic ranges, were designed, built and tested. This versatility was possible because with split inteins you can convert any protein to a controller molecule thanks to their small size, flexibility and modularity 4/5