Aaron Barnes

Med Micro, public health, enterococci, biofilms, microbiome, microscopist, clinical pathology, #doubledoc #postdoc. All tweets are my opinions only.

[Aaron Barnes @MicroTolo]

@jdpereira @BethBGenetics Agree that qPCR is both useful and a good tool; disagree that RNAseq analysis is improved by use of a "gold standard" that is frequently more susceptible to bias (in many forms) than RNAseq itself.

Reasonable minds may differ, depending on the exact nature of their question.

[Aaron Barnes @MicroTolo]

@jdpereira @BethBGenetics I have two ways to measure the length of a room: a calibrated laser rangefinder and a warped wooden meter stick with half the markings rubbed off.

I can use both methods to obtain an answer, but exactly how useful is the meter stick in this case?

[Aaron Barnes @MicroTolo]

@Randy_Au The Fine French Wine Fallacy:

Q: If you take a barrel of fine French wine and add a cup of crap to it, what do you have?

A: The same thing you'd have if you took a barrel of crap and added a cup of fine French wine.

Once you add crap, it's all crap.

[Aaron Barnes @MicroTolo]

@rocza "Save us from able-bodied white men who just can't handle facts" fits on a T-shirt pretty well.🤔

Just saying.

[Elizabeth (Beth) Burton @BethBGenetics]

THERE’S NO REASON TO VALIDATE YOUR RNA-SEQ EXPERIMENT WITH qPCR. RNA-SEQ IS A FAIRLY UNBIASED APPROACH TO ASSESS TRANSCRIPT QUANTITY ACROSS THE WHOLE GENOME AND DOESN’T SUFFER FROM PROBE BIAS THAT CAN BE EXPERIENCED WITH QPCR PRIMERS

[Aaron Barnes @MicroTolo]

@the_transit_guy 🤣

[Aaron Barnes @MicroTolo]

@Merz EXACTLY.

"I'll take 'Things we already knew' for $500, please". 🤣

[Aaron Barnes @MicroTolo]

@S__Baksh @RiderToast Best advice I ever got in med school: "On the wards you can not eat, or you can not sleep, but you can't not eat AND not sleep."

[Aaron Barnes @MicroTolo]

@forensictoxguy

[Aaron Barnes @MicroTolo]

@gingerest @ProfLikeSubst I think you misspelled "the U.S. healthcare 'system' is a Gordian Knot that often seems custom-made to drive good people into a rage when they try to understand it, use it, or even think about it for more than a few minutes at a time."

😉🙃

[Aaron Barnes @MicroTolo]

@drugmonkeyblog 🤯

[Aaron Barnes @MicroTolo]

@textfiles The acquisition of wisdom is often temporarily uncomfortable.

-- Lao-Tzu (probably)

[Aaron Barnes @MicroTolo]

@MarkHoofnagle Seems like a metaphor for *most* things these days...... 😬

[Aaron Barnes @MicroTolo]

@steubens7 @textfiles Username checks out.

[Aaron Barnes @MicroTolo]

@pastramimachine Green Hat Hackers™️

🤣

[Aaron Barnes @MicroTolo]

@MCBazacoPhD

[Aaron Barnes @MicroTolo]

@forensictoxguy 🔥

[Aaron Barnes @MicroTolo]

@garysimonds6 That's excuse number .....five, right? Or six? 🙄

[Aaron Barnes @MicroTolo]

@Rubenstein_Lab Awe. Struck. 🔥