Perttu Permi

PhD, structural biologist, NMR enthusiastic, interested in IDPs, pathogen-host interactions and antibiotic resistance. JyU&UH employ me, but views are my own.

[Perttu Permi @PermiPerttu]

Consequently, we propose re-deliniating repeat boundaries in EspF accordingly.

But there’s more into it, if interested in, see the paper to find out

[Perttu Permi @PermiPerttu]

Happy to share our latest work “Intrinsically disordered enteropathogenic E. coli EspF exploits motif mimicry in high-affinity binding to neural Wiskott-Aldrich syndrome protein and sorting nexin 9”.
#NMR #ecoli #ehec #epec #NMRchat

https://t.co/qJE3GtLxyZ

[Perttu Permi @PermiPerttu]

Also the SNX9 SH3 binding site in EspF has evolved to prevail in competition. Indeed, residues N-terminal to the consensus +xϕPxxP establish an extended interaction and are essential to strong binding. Therefore, we propose to define the SNX9 SH3-binding epitope as ϕxPxRxAPxxP.

[Perttu Permi @PermiPerttu]

We show that single-repeat EspF can outcompete the autoinhibitory N-WASP C-helix and stimulate actin polymerization. EspF uses C-terminal polar interactions to acquire an EspF(U)-like nM affinity for (N)-WASP GBD.

[Perttu Permi @PermiPerttu]

In this work we characterized the structure, dynamics, and binding thermodynamics of EspF and its SNX9 SH3 and N-WASP GBD complexes.

[Perttu Permi @PermiPerttu]

Interested in carrying out PhD studies on S. aureus, IDPs, structural biology, biochem & phys or NMR method development? Apply to an open PhD position in our Lab at the Dept of Chemistry @uniofjyvaskyla DL April 22. #AMR #Staph #MRSA #NMR #NMRchat #NMRjobs https://t.co/y1OtkVNCiI

[Perttu Permi @PermiPerttu]

Happy to share our work now published in @eLife
We show that peptidoglycan (PG) hydrolases LytM and lysostaphin prefer the D-Ala-Gly cross-linked part of PG but unlike lysostaphin, LytM also acts as a D-alanyl-glycine peptidase. @jyunsc #NMR #AMR
https://t.co/SQKwIOBqgd @eLife

[Perttu Permi @PermiPerttu]

The honorary speaker, the Nobel Prize winner @MortenMeldal starts the second day of 20th #nanosciencedays conference @jyunsc @jyuscience with the fascinating and inspiring talk on the click chemistry.

[Perttu Permi @PermiPerttu]

@v_paavilainen @BIOTECH_UH @HiLIFE_helsinki @helsinkiuni Indeed! The Viikki NMR facility has been conducting research and offering services for the scientists over 25 years. Sorry to see it go, but hopefully the 850 MHz system will find a good home at @jyunsc @uniofjyvaskyla

[Perttu Permi @PermiPerttu]

850 MHz NMR lift off from Viikki @BIOTECH_UH @HiLIFE_helsinki @helsinkiuni #nmrchat #nmr

[Perttu Permi @PermiPerttu]

@OnsideWithJouni Ansons ja MacFarlane olisivat viheltäneet😅

[Perttu Permi @PermiPerttu]

Interested in working with S. aureus or IDPs and carrying out studies on structural biology, biochem & phys and NMR method development? You can apply to PhD student positions now open at the Dept of Chem @JyUscience #AMR #Staph #IDP #NMR #NMRchat #NMRjobs https://t.co/fIKAnsntdu

[Perttu Permi @PermiPerttu]

I am thrilled to share the news that our helium liquefier system is up and running, preserving helium also of our #NMR instrumentation @jyunsc

[Faculty of Mathematics and Science @jyuscience]

Katoava luonnonvara kiertoon! Uudella heliumin kierrätyslaitteistolla on tarkoitus saavuttaa yli 90%:n kierrätystehokkuus heliumin suhteen. Rahallisten säästöjen lisäksi sen kierrättäminen on tärkeää kestävän kehityksen kannalta. ⏩ https://t.co/3lNNODZ60q
@jyflacclab @jyunsc

[Perttu Permi @PermiPerttu]

@kuplassasha Liittyneekö meneillään oleva(a)n suure(e)n ja mahtava(a)n junioriorganisaation uudistustyöhön? 🤔

[Perttu Permi @PermiPerttu]

This is certainly great news! https://t.co/TDK2Hekh3K

[Perttu Permi @PermiPerttu]

👀

[NHL.com @NHLdotcom]

Blue Jackets fire Jarmo Kekalainen as GM ⤵️https://t.co/YWiNkAp7Sg

[Perttu Permi @PermiPerttu]

@OnsideWithJouni Itse muistan lapsuudesta hämäristä joskus 80-luvulta ”case Simo Saarisen”, taisi olla Izvestija-turnaus, jossa siirretyn rangaistuksen aikana ruotsalaispelaaja löi kiekon Saarisen mailan kautta tyhjään rysään.

[Helena Aitio @AitioHelena]

Our latest studies on the substrate specificities of S. aureus peptidoglycan hydrolases lysostaphin and lytM is now on BiorXiv. https://t.co/xKe0Ai5I0W

[Perttu Permi @PermiPerttu]

We also show that deviating efficiencies towards S. aureus strains with Epr/Lif factors inserting serines into peptidoglycan cross-bridge as well as FemX/A/B deletions shortening the glycine cross-bridge, can be explained by different substrate specificities of these enzymes.

[Perttu Permi @PermiPerttu]

Our new preprint related to #Staph is now out! We studied the substrate specificities of two peptidoglycan hydrolases, Staphylococcus aureus LytM and S. simulans lysostaphin using #NMR spectroscopy.

https://t.co/6MRbMNuBBb

[Perttu Permi @PermiPerttu]

However, hydrolysis of peptidoglycan fragments devoid of D-Ala-Gly cross-linking are generally 5-10-fold slower, suggesting that LytM also targets the cross-linked, mature peptidoglycan.