Picotti Lab

Mass spectrometry-based #proteomics lab at @IMSB_ETH studying protein conformational changes & their effect on cell physiology. Tweets are from lab members

[Pedro Beltrao @pedrobeltrao]

Thank you European Proteomics Association for the JPA Proteome Pioneer Award in recognition for our group's work in this field. The award promotes "the principles of sharing and integrating resources for the development of excellent research" which is something we do strive for.

[Picotti Lab @Picotti_Lab]

Our paper describing a new method (FLiP-MS) for the global profiling of protein complex dynamics is out @NatureBiotech !

Read here👉: https://t.co/JZBYNzey1G

🔬 Highlights:

- Uses an experimental library of peptide markers integrated with a structural proteomics workflow.

- Probes the assembly state of hundreds of protein complexes simultaneously across conditions directly from whole cell extracts, informing on binding interface occupancy.

- Supports the structural modelling of protein complexes and the design of compounds targeting them

Congratulations to shared first authors @CathyMarulli and Christian Dörig and thanks to our collaborators @LabBarral and @TorstenSchwede lab

@ETH_en #Proteomics #PPIs #SystemsBiology #LiPMS #teammspec #DrugDiscovery

[Picotti Lab @Picotti_Lab]

@labs_mann @ETH_en https://t.co/yuqacI9yuG

[Picotti Lab @Picotti_Lab]

We are proud to announce that Ruedi Aebersold, founder of our institute and beloved colleague, will be awarded the Dr H.P. Heineken Prize for Biochemistry & Biophysics 2024. He receives it together with @labs_mann for their groundbreaking contributions to proteomics @ETH_en

[Picotti Lab @Picotti_Lab]

We found novel candidate interactors for the monomeric and amyloid fibrillar forms of #AlphaSynuclein and for different conformational states of Rab GTPases.

A great collaboration with the @pedrobeltrao, @KorkhovLab Roland Riek and Sean Munro groups.

[Picotti Lab @Picotti_Lab]

Our paper describing a method to screen for conformation-specific protein-protein interactions is out @MolSystBiol.

Read it here: https://t.co/YPPjA8w6BA

The approach can also be used to map interaction sites.

@IMSB_ETH @ETH @ETH_en
#proteomics #massspectrometry #interactomes

[Picotti Lab @Picotti_Lab]

Congratulations, Albert. An exciting research area you are pioneering. We look forward to exciting discoveries ahead.

[Picotti Lab @Picotti_Lab]

High resolution protein complex analysis beyond AP-MS by the Gstaiger group: https://t.co/TbyUAmgJOU.

Nice collaborative adventure at @IMSB_ETH from the @MGstaigerlab members @FabianFrommelt , @anfossati , @federico_uliana and from @bwollsch lab members

[Michael Hengartner @m_hengartner]

Exciting development for 🇨🇭 research!

Following a three-year hiatus, scientists from Switzerland can now participate in the 2024 ERC Advanced Grants.

The ETH Board wishes that this solution can be extended to all 2025 calls in summer 2024.

#HorizonEurope

[NCCR RNA & Disease @NCCR_RNADisease]

Publication by the Allain & Leitner Labs (@IMSB_ETH, @ETH_en) with 1st authors Tebbe de Vries & Mihajlo Novakovic (@tflut1,@Mehighlow993) "Specific #protein-#RNA interactions are mostly preserved in biomolecular #condensates" @ScienceAdvances
https://t.co/L9mEDq7A4B
#MS #LLPS

[Mol Syst Biol @MolSystBiol]

N&V by @seth_margolis on the study by @Picotti_Lab & @MichalSharon3 developing an advanced #proteomics method (PiP-MS) to comprehensively explore 20S #proteasome substrates in the human proteome ➡️ https://t.co/0m8jLPboir

[Picotti Lab @Picotti_Lab]

Our warmest congratulations to Franziska Elsaesser on the successful defense of her PhD.

Excellent job, Franzi!

[Picotti Lab @Picotti_Lab]

Great talk by Tetiana Serdiuk at the AD/PD conference on biochemical differences between amyloid strains linked to different alpha-synucleinopathies. @adpdnet

[Picotti Lab @Picotti_Lab]

This study is the result of an excellent collaboration with the @wolfgangkhuber lab, @ArosioLab_ETH, @EWB_Lab, @BrittaVelten lab and @HeatherDMaynard lab!
@IMSB_ETH @ETH @ETH_en @embl #proteomics #massspectrometry #proteinstability #osmolytes

[Picotti Lab @Picotti_Lab]

We also learned about the thermal behavior of intrinsically disordered proteins. Many predicted disordered regions may fold in situ!

[Picotti Lab @Picotti_Lab]

Our paper on the mechanisms of protein thermal stabilization by osmolytes is out @nchembio. We found that osmolytes thermally stabilize many proteins in situ using various mechanisms (but there's a dominant one – read the paper to find out).
Read it here: https://t.co/xU1a4DZ7Y0

[D-BIOL @ ETH Zurich @ETH_DBIOL]

The paper by @KorkhovLab, together with @Picotti_Lab, Alexander Leitner and @federico_uliana, now out in @emboreports, describes the cryo-EM- and structural proteomics-based analysis of adenylyl cyclase AC8 and its macromolecular complexes. More: https://t.co/AkCoQhO9B2

[elevyLab @ElevyLab]

So happy to see our work published at @CellCellPress !

We added the quaternary structure dimension to proteome-wide, #AlphaFold2 predictions:

The published paper: https://t.co/xeGkqNxxvI

The original 🧵 (Two more experimental structures since!)
https://t.co/IsoV7vTXEx

[Mol Syst Biol @MolSystBiol]

A systematic investigation of 20S #proteasome substrates reveals 100s of substrates & differential functionality of naïve and oxidized 20S proteasome complexes ➡️ https://t.co/az9j7kyH1W
@Picotti_Lab @IMSB_ETH @MichalSharon3 @WeizmannScience #proteomics #massspectrometry

[Picotti Lab @Picotti_Lab]

Our paper with @MichalSharon3 is now live @MolSystBiol. We identified hundreds of substrates of the 20S proteasome, enriched in RNA- and DNA-binding proteins with IDRs. A variety of substrates showed site-specific cleavage, suggesting functional products.
https://t.co/YjWeNm6Vcc