PratsaN

[David R. Liu @davidrliu]

Three of the more costly and frequently used reagents in molecular biology are rolling circle amplification (RCA) mix, chemically competent MachOne (or other E. coli) cells, and Gibson assembly mix. Postdoc Julian Willis—who is starting his own lab in the UK soon—developed homemade recipes for each that reduce costs ~3x (or more at large-scale). Links to each recipe:

RCA mix:
https://t.co/Cmir57uM0J

Chemically competent MachOne cells:
https://t.co/kNYVxLIToA

Gibson assembly mix:
https://t.co/OFwXmmLDWB

[Bunpote Siridechadilok @bunpote_s]

OK, quite delayed but my first-ever tweet. Our first paper on CRISPR-Cas.

Potent programmable antiviral against dengue virus in primary human cells by Cas13b RNP with short spacer and delivery by VLP https://t.co/482t8F8QZT