Ramya

Developmental Biologist. RTs are mostly bookmarks for reading later.

[Ramya @RamyaSingh]

@aemonten Yeah it does matter. Conference a would be more likely to attract locals whereas conference b is more likely to have people show up from everywhere.

[PLOS Genetics @PLOSGenetics]

Mutations in GLP-1, a Notch receptor, can result in #tumor formation

Singh et al @dhansen01 show that reduction of Derlin activity suppresses over-proliferation due to GLP-1/Notch mutations in C. elegans
#CancerResearch

🔎 Learn more: https://t.co/nCMHMBxT9b
📷: @RamyaSingh

[Ramya @RamyaSingh]

Very pleased to have been able to support open-access publishing! ❤️ Tagging Twitter-present co-authors @dhansen01 and @Chriswang1689. Thank you reviewers and editors for your time! 🙏🙏🙏 6/6

[Ramya @RamyaSingh]

📣 🎉Very happy to share our work, out now in @PLOSGenetics https://t.co/U5Zj1t13WL. We found that loss of Derlin proteins, best known for their role in ER-associated degradation(#ERAD), suppress Notch-dependent overproliferation in the C.elegans germ line 🎉📣. 1/6

[Ramya @RamyaSingh]

Reducing Derlin activity and/or inducing ER stress could potentially be a way to reduce the effect of Notch(gf)- dependent overproliferation/tumours.💊🚑👩‍⚕️ 5/6

[Sandra Lemke @SandraBLemke]

How do dynamic changes in cell packing shape epithelial tissues during #morphogenesis? Read our review in @CurrentBiology to find out what strategies cells use to create the beautifully diverse shapes of organs. @celestemnelson @EPrinceton @Princeton https://t.co/IFDBqe1WRT

[Jean-Yves Tinevez @jytinevez]

A 2nd post about the new #TrackMate version:
Using TrackMate to segment 3D objects using a slice-by-slice approach.

1/n

[Ramya @RamyaSingh]

@MHendr1cks Absolutely. Building taller buildings might be nice too.

[Ramya @RamyaSingh]

@MHendr1cks I've heard they're more energy efficient though since they allow for daylight to reach the center of the building, reducing lighting/heating costs.

[Dr Sarah McCallum @Sarah_MccGlia]

Fluorescence imagers of Twitter. What's your favourite 5-colour palate for microscopy images. Ideally something colourblind friendly that is clear without single panels #anotherfelllowshipapplication

[Brandon Bright-Barker, PhD✨ @AstroBarker]

@katiemel25 Why print it when you can print it (to a terminal) 🙂 https://t.co/uY6LArmUvX

[microPublication @Micropub7n]

The primordial germ line is refractory to perturbations of actomyosin regulator function in C. elegans L1 larvae #micropublication #biology #data #newfinding #celegans https://t.co/Khz8sgooIJ

[Nat Friedman @natfriedman]

We’ve just added built-in citation support to GitHub so researchers and scientists can more easily receive acknowledgments for their contributions to software.

Just push a CITATION.cff file and we’ll add a handy widget to the repo sidebar for you.

Enjoy! 🎉

[Peter Sobolewski @psobolewskiPhD]

If you want to try @napari_imaging maybe with #clEsperanto or #TensorFlow plus #StarDist NATIVE on your #AppleSilicon Mac, I've finally written up some directions:
https://t.co/yP1Jw25wZZ
Good luck!
#BioImageAnalysis

[Matias Calderini @MatiCalderini]

@Pi_In_The_Sky @matplotlib I am biased but... I think my #matplotlib tutorials are quite good. I should have a pandas one coming out soon as well:

https://t.co/dnWLaL2pkU

https://t.co/VhLN5kON3w

Good luck!

[Ramya @RamyaSingh]

The bulk of my PhD work is out! Characterizing a suppressor of a strong Notch-dependent over-proliferation phenotype (aka tumour) lead us to uncover the role of ER-associated degradation proteins (#Derlin) and the Unfolded Protein Response. #ERAD #UPR #ERstress #stemcells

[bioRxiv @biorxivpreprint]

Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line https://t.co/H2bP201Bjw #bioRxiv

[Ramya @RamyaSingh]

@ShapiroRebecca Also has the miniprep kit been working ok in the lab for others? Can test out the miniprepped DNA by good old RE digest + gel to get a sense of if it looks right or is possibly rearranging.

[Ramya @RamyaSingh]

@ShapiroRebecca How many colonies do you screen/grown up? A colony PCR might enable you to screen a bunch of colonies for the region of interest, before growing it up for a miniprep. Maybe the intact plasmid is there in only a few of the colonies, so more screening might help.