Ana Saemy Chong Lujan

[Ana Saemy Chong Lujan @SaemyChong]

My #RSCPoster is now up on LinkedIn! I'll be happy to answer your questions. 😃
#NanoMat #RSCNano
https://t.co/YUkCai1fG6

[Ana Saemy Chong Lujan @SaemyChong]

"Photodynamic Therapy for pathogenic fungi treatment using graphene oxide as photosensitizer carrier" Graphene-based materials have been used in PDT as a novel way to accelerate photosensitizers reactions to produce cell death @IPN_MX @CicataIPN_Altam @cigmmad #EMDA-4/V26

[Ana Saemy Chong Lujan @SaemyChong]

@TheScienceGoat @LatinXChem @nanoscale_rsc I would use Alexa Fluor, which is a fluorescent probe used for cell bioimaging and to obtain super-resolution.

[Ana Saemy Chong Lujan @SaemyChong]

Hi @LatinXChem, presenting my work "Photodynamic Therapy for pathogenic fungi treatment using Graphene Oxide as photosensitizer carrier" at #LatinXChem23 #LatinXChemNanoMat #NanoMat057 @nanoscale_rsc #Graphene #PDT #ROS #MethyleneBlue

[Ana Saemy Chong Lujan @SaemyChong]

@NanoCriss @LatinXChem @nanoscale_rsc Hi @NanoCriss, thank you! I haven't measured the surface charge of GO yet. It is part of my future work because I expect to employ ELS. Regarding microscopy, I have only used AFM, but I plan to use other techniques in the future.

[Ana Saemy Chong Lujan @SaemyChong]

@LatinXChem @nanoscale_rsc @CicataIPN_Altam @cigmmad

[Ana Saemy Chong Lujan @SaemyChong]

@TheScienceGoat @LatinXChem @nanoscale_rsc 3) Epifluorescence and confocal microscopy assess and quantify cellular damage. Epifluorescence identifies damaged cells through specific stains, while confocal offers 3D visualization. Both techniques allow the observation of morphological changes.

[Ana Saemy Chong Lujan @SaemyChong]

@TheScienceGoat @LatinXChem @nanoscale_rsc 2) We used a red LED with a wavelength of 660 nm. Samples were subjected to 5 minutes of continuous irradiation.

[Ana Saemy Chong Lujan @SaemyChong]

@TheScienceGoat @LatinXChem @nanoscale_rsc Hi @TheScienceGoat, Thanks!
Answering your questions:
1) MB can be used for both purposes, but in this project, we are primarily studying its effect as a photosensitizer. Staining was included as an additional experiment to assess cell damage.

[Ana Saemy Chong Lujan @SaemyChong]

Hi @LatinXChem, presenting my work "Photodynamic Therapy for pathogenic fungi treatment using Graphene Oxide as photosensitizer carrier" at #LatinXChem23 #LatinXChemNanoMat #NanoMat057 @nanoscale_rsc #Graphene #PDT #ROS #MethyleneBlue

[Ana Saemy Chong Lujan @SaemyChong]

@cigmmad Of course. We would only have to control the interval between each session and the number of follow-up sessions so that the treatment is better for the patient.

[Ana Saemy Chong Lujan @SaemyChong]

Use of graphene-based photoactivators for photodynamic therapy as an alternative against onychomycosis. #RSCPoster #RSCMat #RSCNano #RSCEng