CRISP_SCIENCE

RIKEN (CODATA, WDCM ...) - NIG (CODATA, DDBJ/CIB, WDCM, OECD BRC, GBIF, JST BIRD, TPRP ...) - FREE

[CRISP_SCIENCE @ScienceCrisp]

"Self-Assembled Skin Equivalents with Monoclonal #CRISPR/Cas9-Modified N/TERT-1 Keratinocytes: A Cutting-Edge model for Human Skin and its Diseases"
Marta Slaufova, Tugay Karakaya, Michela Di Filippo, Thomas Kündig, Hans-Dietmar Beer.
Adv Heakthcare Mater. 2026-05-26.
https://t.co/XG12cMxaK4

[CRISP_SCIENCE @ScienceCrisp]

REVIEW "Evolving strategies for lineage tracing: Genetic markers, synthetic barcodes, and natural variants"
Zhixin Kang, Hui Chen, Siyang Li, Shou-Wen Wang, Bin Zhou.
Cell Stem Cell. 2026-05-29.
https://t.co/sgRnPJ915T

[CRISP_SCIENCE @ScienceCrisp]

"Identification of Closantel as a small-molecule inhibitor of the compact #CRISPR-Cas RNA editor Cas13bt3"
Xulong Chen Binbin Zhan, Ruyi Shi, Jingxuan Chen, Zhonghui Lin, Zuoan Li.
Mol Divers. 2026-05-27.
https://t.co/GISicWQaMC

[CRISP_SCIENCE @ScienceCrisp]

"An optimised lipid nanoparticle platform enables efficient #CRISPR/Cas9 genome editing in hard-to-transfect cells"
Xinpu Yang, Rui Sang, Gyorgy Hutvagner, Alex W Hewitt, Diangeng Li, Yi Li, Wei Deng.
Acta Biomater. 2026-05-28.
https://t.co/9xl22ZH5E8

[CRISP_SCIENCE @ScienceCrisp]

"Double-#CRISPR Knockout Simulation (DKOsim): A Monte-Carlo randomization system to model cell growth behavior and infer the optimal library design for growth-based double knockout screens"
Yue Gu, Traver Hart, Luis Leon-Novelo, John Paul Shen. PLoS Comput Biol. 2026-04-17. https://t.co/jmxzY8VqmX

[CRISP_SCIENCE @ScienceCrisp]

"Engineering herbicide-resistant sorghum with #CRISPR/Cas9-mediated adenine base editing"
Jianshuang Zhou, Ruirui Li [..] Jinjie Zhu, Qian Qian, Baoqing Dun.
J Integr Plant Biol. 2026-05-18.
https://t.co/ubAnaTtHdU
ABE8e; SbALS<W545R>

[CRISP_SCIENCE @ScienceCrisp]

“Uncleavable fusion of Csy4 with prime editors for low Csy4 toxicity and highly efficient prime editing in Arabidopsis”
Wei Sun, Yu Lu, Zhenghong Cao [..] Qi-Jun Chen.
Plant Physiol. 2026-05-06.
https://t.co/nb0suHNhvq
The sufficient protection of the 3′ end of pegRNAs by the pegRNA-Csy4RS-Csy4 complex accounts for the high-efficiency of Csy4-based PEs. However the group observed strong Csy4 toxicity in plants including maize and Arabidopsis.

[CRISP_SCIENCE @ScienceCrisp]

Review "Split crRNAs Enhance Cas12a Diagnostic Performance"
Xinyi Guo, Tian Tian, Xiaoming Zhou.
ChemBioChem 2026-05-17.
https://t.co/1OF15czPKw
#CRISPR Dx

[CRISP_SCIENCE @ScienceCrisp]

"Parallel gene amplification by Cas9 nickase for generating functionally heterogeneous cell populations"
Hiroaki Takesue, Satoshi Okada, Takashi Ito.
Cell Rep Methods. 2026-05-25.
https://t.co/yud9cHoWo6

#CRISPR

Parallel BITREx (Break-Induced Replication-mediated gene amplification) generates functionally heterogeneous cell populations, enabling the systematic exploration of optimal gene dosages for enhanced biological functions.

It helps identify elite yeast genotypes for optimized β-carotene production.

[CRISP_SCIENCE @ScienceCrisp]

"Mismatch type impacts interference and priming activities in the type I–E #CRISPR–Cas system"
Phong T Phan, Meric Ozturk [..] Dipali G Sashital.
J Biol Chem 2026-05-25.
https://t.co/1KZ6fMViRw

[CRISP_SCIENCE @ScienceCrisp]

"In vivo systematic detection of the outcomes of #CRISPR-Cas9-mediated DNA repair in skeletal muscle stem cells"
Liangqiang He, Yang Fu, Ziliu Wang, Qin Zhou, Hao Sun, Huating Wang.
Mol Ther. 2026-04-20.
https://t.co/ZiP7CAWPcJ

[CRISP_SCIENCE @ScienceCrisp]

"Burkholderia vietnamiensis genes involved in extracellular medium-chain-length polyhydroxyalkanoate (mcl-PHA) degradation"
Zhong Ling Yap [..] Silvia T Cardona.
Appl Environ Microbiol. 2026-04-03/04-22.
https://t.co/AfZhwtDiLr
Bioplastics (such as mcl-PHA) represent promising alternatives to petroleum-based plastics, yet their biodegradation remains insufficiently understood.

[CRISP_SCIENCE @ScienceCrisp]

“DUSP11 is an RNA triphosphatase that limits PspCas13b activity by destabilizing gRNA abundance in mammalian cells"
Jacob Purcell [..] Joseph Rosenbluh.
Nucleic Acids Res. 2026-05-04.
https://t.co/uF5DzUDLT2
#CRISPR-Cas9 KO screen
… DUSP11 KO increased gRNA levels 2.5–4-fold and enhanced PspCas13b-mediated knockdown across multiple cell lines.

[CRISP_SCIENCE @ScienceCrisp]

"#CRISPR-Mediated Intronic Knockin of Pre-amiRNA Enables Targeted Gene Silencing"
Jinsong Dong, Ke Zhang, Minglei Song [..] Mingguang Lei.
Plant Commun. 2026-05-14.
https://t.co/LhctVHLxRf
amiRNA (artificial microRNA)

[CRISP_SCIENCE @ScienceCrisp]

“Tuning mitotic recombination with patterned DNA nicks for precision mosaic analysis."
Yifan Shen, Ann T. Yeung [..] Chun Han.
Proc Natl Acad Sci U S A. 2026-05-27/Jun.
https://t.co/bbfjCMe4bg
＃CRISPR
MAGIC（mosaic analysis by gRNA induced crossing-over) based on Cas9
> Nickase-MAGIC based on Cas9 nickase
(safer, more efficient & less off-target)

[CRISP_SCIENCE @ScienceCrisp]

Review "Genome engineering of plant photosynthesis for carbon sequestration"
Evan D. Groover, Flora Z. Wang [..] David F. Savage, Krishna K. Niyogi.
Nat Rev Bioeng 2026-05-26. https://t.co/I7hFqej0MP
incl. #CRISPR GE

[CRISP_SCIENCE @ScienceCrisp]

NEWS "PRINCE: A Small-Molecule Switch for Safer Gene Editing”
Genetic Engineering & Biotechnology News. 2026-05-28. https://t.co/ycyPn6qsCT

[CRISP_SCIENCE @ScienceCrisp]

“Coordinated regulation using small-molecule drugs enables controlled therapeutic genome editing and enhanced genomic precision in situ"
Ju Zhang Li Chen, Xingyu Zhu[..] Jiaxu Hong, Yu Wang.
Sci Transl Med. 2026-05-27.
https://t.co/uy9YlYu71C
＃CRISPR
PRINCE (Cas9) & Little Prince (Cas12i & compact Cas12 variants)

[CRISP_SCIENCE @ScienceCrisp]

“Coordinated regulation using small-molecule drugs enables controlled therapeutic genome editing and enhanced genomic precision in situ"
Ju Zhang Li Chen, Xingyu Zhu[..] Jiaxu Hong, Yu Wang.
Sci Transl Med. 2026-05-27.
https://t.co/uy9YlYu71C
＃CRISPR
PRINCE (Cas9) & Little Prince (Cas12i & compact Cas12 variants)

[CRISP_SCIENCE @ScienceCrisp]

“Application of an endogenous pGhαGloA promoter in the #CRISPR/Cas12a system for efficient genome editing to create glandless cotton germplasm”
Chenyu Li, Zumuremu Tuerxun[..] Shuangxia Jin, Bo Li.
J Integr Agric 2026-04-10.
https://t.co/TM06dntFTE
… the editing efficiencies at 2 sites were up to 88.24% and 93.37%.

[CRISP_SCIENCE @ScienceCrisp]

"Treatment of Shiga toxin–producing E. coli infection by #CRISPR-Cas–targeted cleavage of the Shiga toxin gene in animal models"
Galtier M, Krawczyk A [..] Duportet X, Bikard D, Fernandez-Rodriguez J.
Sci Transl Med 2026-05-27.
https://t.co/XozgJKnyBO