Robert F. Kennedy Jr. might be our next HHS secretary. We really are about to trade the Cancer Moonshot and Alzheimers research program for a focus on why seed oils and fluoridated water are bad. This truly is the darkest timeline.
@iaahmed123 NP40 buffers without SDS will lyse cells but not nuclei. Not sure how well the membrane fraction specifically is recoverable but I can send you a recipe if you DM me.
@PaulCMcKeePsych If you're looking for an NINDS DSPAN application, I'm happy to share. DM me your email address.
@crmcdermott @___AshleyH, your apps are a little more current. Care to share?