The Ashe lab

BMP signalling, transcription and stem cells in Drosophila. University of Manchester 🪰 Lab account

[The Ashe lab @TheAsheLab]

We have used different MiMIC stocks to show how this approach can be used to visualise transcription of different genes in tissues including the embryo, larval wing disc, brain and ovary. Here the movie shows Daughters against dpp (Dad) transcription in follicle cells:

[The Ashe lab @TheAsheLab]

Looking for a quick way to endogenously tag genes in Drosophila to live image transcription? Then check out our paper in @Dev_journal for simply and rapidly inserting MS2/PP7 cassettes into any of the thousands of genes carrying a MiMIC insertion.

https://t.co/dWxwkbwP1g 🪰

[The Ashe lab @TheAsheLab]

The second is investigating the mechanism of cell signalling by cytonemes in Drosophila ovarian germline stem cells: https://t.co/SVGBtZsPkr

Deadline 31/01/25 🧫

(2/2)

[The Ashe lab @TheAsheLab]

TWO fully-funded PhD studentships available in the lab! Please RT and share 🧬

The first investigates the role of cytoplasmic P-bodies in the robustness of embryonic development: https://t.co/sdr9XF0UxU

Deadline 10/01/25 🪰

(1/2)

[The Ashe lab @TheAsheLab]

Finally, we find that a protostome-specific C-terminal Tsg tail is likely to be an intrinsically disordered region and is essential to full Tsg action in regulating dorsoventral patterning in the Drosophila embryo.

[The Ashe lab @TheAsheLab]

Excited to share our latest findings on the extracellular regulation of BMP signalling by Twisted gastrulation! We identify how Tsg binds the Chordin family of BMP antagonists, leading us to propose an avidity-driven mechanism of BMP signalling control. https://t.co/tizEHfV7zq

[The Ashe lab @TheAsheLab]

Based on these findings we propose an avidity-driven mechanism of extracellular BMP control by Tsg. By binding BMP via its N-terminal domain, and Sog via its C-terminal domain, interaction at one site could then make other interactions more likely through cooperative binding.

[The Ashe lab @TheAsheLab]

Thank you to everyone who supported our Christmas collection again this year, all the goodies have been dropped off with the wonderful folk at @EmmelinesNew 🎁 for any last minute donations please see their Amazon wishlist:

https://t.co/NoaTPeSy0i

🎄@MCF_UoM
🎄@FBMH_DocAcad

[The Ashe lab @TheAsheLab]

In this work, we show that differentiating germ cell cysts extend cytonemes to reactivate BMP signalling, recolonise an empty niche and dedifferentiate to germline stem cells.

[The Ashe lab @TheAsheLab]

Excited to share our latest preprint in collaboration with @WilcocksonScott, which describes the mechanism of germ cell dedifferentiation in the Drosophila ovary: https://t.co/iABee9APVh

[The Ashe lab @TheAsheLab]

*Diwali!

[The Ashe lab @TheAsheLab]

Fabulous Divali celebrations again this year in the lab. Thank you @Nabarundevbio for spoiling us 🪔

[The Ashe lab @TheAsheLab]

It’s that time of year! 🎅🏼 We are collecting donations again for the wonderful @EmmelinesNew throughout November. See our flyer below for details of required items and where to bring them 🎁

@MCF_UoM @FBMH_UoM

[Anzy Miller @AnzyMiller]

Are you interested in exploring the interplay between dynamic protein expression and cell division, and how they impact fate decisions in neural development? We are looking for a PhD student to combine live imaging, optogenetics and data modelling. @BigaVeronica @TheAsheLab

[Yad Ghavi-Helm 🐾 @yad_ghavi]

📢We are recruiting!
Fully funded Postdoctoral position to work on enhancer-promoter interactions in the Drosophila embryo in collaboration with @drosoigmm
@IGFLyon @ENSdeLyon
#drosophila #3Dgenome #enhancer #transcription

[The Ashe lab @TheAsheLab]

A great few days with @yad_ghavi lab on our joint retreat in Lyon brainstorming and hiking in the countryside. Thanks to everyone for their hospitality and organisation!

Of course Colin joined us on our train journey in line with our commitment to sustainability and cake! 🍃