Redness highly observed on trtmnt with 3% ANS, why no shooting?
Production of anthocyanin, a secondary metabolite, requires additional energy than primary metabolism (chl production) as it is not part of the main plant life machinery, let alone stimulated in a callus
Absorbance at pH 1 where the cyanidin intermediate is protonated and absorbance at approximately pH 4 where it is deprotonated, the difference will be multiplied to the molecular weight of the cyanidin, and dilution factor used
If the chlorophyll mask the anthocyanin pigment at optimal pH and anthocyanin is red at acidic env, then subjecting the calli in lower pH buffer solution might detect the cyanidin intermediates ๐ค๐ค๐ค