Tyler Smith

Instructor for TA Training and Introductory Biology | MIT Biology

[Tyler Smith @ToxoTyler]

Very cool to see our @LouridoLab work and the work of @ElenaJmnezRz & @MeissnerLab colleagues highlighted by @AulieTsee and @drchristonkin. Excited to see where these high-throughput phenotyping platforms go next

[Trends in Parasitology @TrendsParasitol]

Drs @AulieTsee & @drchristonkin highlight two recent @NatureMicrobiol articles from @LouridoLab @MeissnerLab @ElenaJmnezRz & colleagues in this Spotlight: A #CRISPR upgrade unlocks #Toxoplasma gene function. @WEHI_research @WhiteheadInst @LMU_Muenchen

https://t.co/hWdS52JKPR

[Tyler Smith @ToxoTyler]

Welcoming my new work from home companion, Cali, to the family <3

[Tyler Smith @ToxoTyler]

Finishing up cleaning out my lab space and found this gem from 2017. TS lab notebook 1, page 1

[Tyler Smith @ToxoTyler]

Happy to share that in August I will be joining @MITBiology as an instructor for TA training and introductory biology, working with an exciting team to help develop curriculum and pedagogy strategies!

[Elena Jiménez-Ruiz @ElenaJmnezRz]

I’m very happy to announce that our paper on how we found 2 key factors involved in #toxoplasma egress thanks to a newly stablished splitCas9 screen is out now in @NatureMicrobiol. @FelixStortz @PerizJavier @GrechJanessa @MeissnerLab https://t.co/CoOal6yaOg

[Sebastian Lourido @LouridoLab]

Just days after introducing Dr. @ToxoTyler, I get to share his magnum opus in its final form: "Screening the #Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress" https://t.co/Tl7AfQGcWp

[Whitehead Institute @WhiteheadInst]

.@LouridoLab developed a new method to probe gene function in the parasite Toxoplasma gondii and uncovered a previously unstudied kinase, SPARK, linked to the parasites' ability to enter and leave host cells: https://t.co/2q2F3x6umf

[Tyler Smith @ToxoTyler]

It's been an eventful but very exciting week!

[Nature Microbiology @NatureMicrobiol]

OUT NOW👉Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress

A work by Lourido & colleagues @LouridoLab @WhiteheadInst @ToxoTyler

Read it here: https://t.co/kPvdtaRr8M

https://t.co/Vn8I2GsB0J

[Tyler Smith @ToxoTyler]

@SivaSankariIMS Thanks Siva! Yes, it shouldn't be a problem!

[Tyler Smith @ToxoTyler]

6 years in the making, I'm defending on April 25th. Message me if you would like zoom details!

[Tyler Smith @ToxoTyler]

@scatter_plot thanks Gina! hope you're doing well :)

[Tyler Smith @ToxoTyler]

@danpbollen Pure skill

[Tyler Smith @ToxoTyler]

If you sit in the middle of the 3 bench spots at a train stop during a pandemic, just why?

[Tyler Smith @ToxoTyler]

Most of my TC habits still come from my former rotation mentor 5 years ago

[Diego Huet @HuetLab]

Please RT📢! Come join our young and growing team (https://t.co/b5cVC9NMFU) located in the @CTEGD. We are looking for a postdoc to work on the unique organellar biology of Toxoplasma gondii! 🔬🥼🧫🦠
More details here: https://t.co/yKsHQGfi8E

[Tyler Smith @ToxoTyler]

@ClareHardingLab @LouridoLab Thanks Clare :) I always took inspiration from the toxo microscopy you used to do in the lab

[Tyler Smith @ToxoTyler]

@BenManOfWald Thanks Ben :) you can take the mate out of the bay, but you can’t take the bay out of the mate #BayOne4Lyfe

[Tyler Smith @ToxoTyler]

Finally, in this work we generated a 1160 clone array, which would not have been possible without a team effort. I am grateful to be in a lab where others are so willing to help achieve crazy ideas. Here’s some pics of us rearraying >50 plates of subclones over two mornings (4/4)

[Tyler Smith @ToxoTyler]

Very excited to share our work! We developed the HiT system for HR-based screens in both arrayed and pooled formats with spatiotemporal resolution. We profiled T. gondii’s kinome and uncovered SPARK, a novel regulator of parasite invasion and egress. https://t.co/lRnJwiHBEg (1/4)

[Tyler Smith @ToxoTyler]

I would like to highlight the works of @LopezGabriellaS who was critical in the development of the HiT vectors and @e_shortt for the generation of scarless CDPK1 and 3 clones. And of course @LouridoLab for his continuing tireless mentor ship (3/4)